Human PGLYRP1 ELISA Kit PicoKine®

Product Name

Human PGLYRP1 ELISA Kit PicoKine®

View all PGLYRP1/PGRP-S ELISA kits

SKU/Catalog Number

EK2267

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PGLYRP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PGLYRP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PGLYRP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2267)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q22-P196

Sensitivity

<150 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2267 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2267 is reactive to PGLYRP1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2267 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PGLYRP1/PGRP-S

Peptidoglycan recognition protein 1, PGLYRP1, also known as TAG7, is an antibacterial and pro-inflammatory innate immunity protein that in humans is encoded by the PGLYRP1 gene. Peptidoglycan recognition proteins (PGRPs) are molecules that recognize peptidoglycan, a large component in bacterial cell walls. In insects, PGRPs activate antimicrobial pathways, and in mammals PGRPs function as antibacterial neutrophil proteins. PGRP-L halts bacterial growth by acting as an alanine amidase, an enzyme that hydrolyzes the amide bond of bacterial peptidoglycan. PGRP-I and PGRP-I∫ are also members of the PGRP family that help recognize bacteria by binding to peptidoglycan and Gram-positive bacteria, but they do not have amidase activity. PGRP-S participates in intracellular killing of Gram-positive bacteria by stimulating two antimicrobial defense systems, the prophenoloxidase cascade and the antimicrobial peptides through Toll receptors.

Kit Components

Catalog Number	Description	Quantity
EK2267-CAP	Anti-Human PGLYRP1 Pre-coated 96-well strip microplate	1
EK2267-ST	Human PGLYRP1 Standard	2 vials, 20 ng/tube
EK2267-DA	Human PGLYRP1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	312	625	1,250	2,500	5,000	10,000	20,000
O.D.	0.025	0.042	0.098	0.169	0.398	0.741	1.298	1.987

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PGLYRP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PGLYRP1/PGRP-S in ELISA kits from four different production batches/lots.

Gene/Protein Information For PGLYRP1 (Source: Uniprot.Org, NCBI)

Alternative Names

peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PGLYRP; PGLYRP1; PGRPpeptidoglycan recognition protein; PGRPS; PGRP-S; PGRP-SMGC126894; PGRPSMGC126896; TAG7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L PGLYRP1 PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L peptidoglycan recognition protein 1 peptidoglycan recognition protein 1|TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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