Product Info Summary
SKU: | EK7005 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
Product info
Product Name
Human Testosterone(T) ELISA Kit (Competitive ELISA)
SKU/Catalog Number
EK7005
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Testosterone (T) 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Testosterone(T) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7005)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.1 ng/ml
Assay Range
0.2-80 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7005 is reactive to in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7005 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Goat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate |
Human Testosterone (T) Standards (S0~S5) | 6 | 0.5ml | Testosterone (T) (0, 0.2, 1, 4, 15, 80 ng/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300 |
HRP Conjugated antigen | 1 | 6ml | HRP Conjugated Human Testosterone (T), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300 |
Antibody | 1 | 6ml | Rabbit anti- Human Testosterone (T) Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue |
Controls | 2 | 0.5ml | Testosterone (T), 100% natural protein, 0.1% Proclin-300 Range of Control 1: 2.1-4.9 ng/mL Range of Control 2: 9-21 ng/mL |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Human Testosterone(T) ELISA Kit standard curve
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Protein Target Info & Infographic
Gene/Protein Information For (Source: Uniprot.org, NCBI)
Gene Name
Full Name
Weight
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Specific Publications For Human Testosterone(T) ELISA Kit (Competitive ELISA) (EK7005)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Human Testosterone(T) ELISA Kit (Competitive ELISA)
Question
What kind of diluent should be used for plasma samples using EK7005?
Verified customer
Asked: 2020-11-24
Answer
For the Human Testosterone(T) ELISA Kit (Competitive ELISA) (EK7005), 1% BSA in PBS can be used to dilute plasma samples.
Boster Scientific Support
Answered: 2020-11-25
Question
What kind of diluent is for the standard in EK7005?
Verified customer
Asked: 2020-11-24
Answer
The standards in the Human Testosterone(T) ELISA Kit (Competitive ELISA) (EK7005) are ready to use and it is unnecessary to dilute them.
Boster Scientific Support
Answered: 2020-11-25
Question
Can mouse plasma be tested by EK7005?
Verified customer
Asked: 2020-09-08
Answer
Yes, the Human Testosterone(T) ELISA Kit (Competitive ELISA) (EK7005) could be used for mice plasma.
Boster Scientific Support
Answered: 2020-09-09