Product Info Summary
SKU: | EK7012 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
Product info
Product Name
Human Thyroxine (T4) ELISA Kit
SKU/Catalog Number
EK7012
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human T4. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Thyroxine (T4) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7012)
Clonality of Antibodies
See Datasheet for details
Sensitivity
15 ng/mL
Assay Range
20-320 ng/mL
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7012 is reactive to in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7012 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Rabbit Anti- T4 Antibody, Polystyrene micro-well plate |
Human T4 Standards (S0~S5) | 5 | 1ml | T4 (0, 20, 40, 80, 160, 320 ng/mL), 100% detoxification serum, 0.1% Proclin-300 |
Avidin-HRP conjugate | 1 | 6ml | Avidin-HRP conjugate, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1% Proclin-300 |
Biotinylated antigen | 1 | 6ml | Biotinylated T4, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300 |
Controls | 2 | 1ml | T4, 100% natural protein, 0.1% Proclin-300 Range of Control 1: 44.3-82.3 ng/mL Range of Control 2: 112-208 ng/mL |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Human T4 ELISA Kit standard curve
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Protein Target Info & Infographic
Gene/Protein Information For (Source: Uniprot.org, NCBI)
Gene Name
Full Name
Weight
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In this infographic, you will see the following information for : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Thyroxine (T4) ELISA Kit (EK7012)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Human Thyroxine (T4) ELISA Kit
Question
Are the standard (S1~ S5) and Biotinylated antigen of EK7012 chemical compounds.
Verified customer
Asked: 2020-10-29
Answer
For the Human Thyroxine (T4) ELISA Kit (EK7012), the standard (S1~ S5) and Biotinylated antigen are chemical compounds.
Boster Scientific Support
Answered: 2020-11-01
Question
The product description of EK7012 says it is 100% natural protein, does it mean it is human serum?
Verified customer
Asked: 2020-10-29
Answer
The Positive control of the Human Thyroxine (T4) ELISA Kit (EK7012) is human serum.
Boster Scientific Support
Answered: 2020-11-01
Question
What is the storage temperature of EK7012?
Verified customer
Asked: 2020-10-28
Answer
Please store the Human Thyroxine (T4) ELISA Kit (EK7012) at 2-8°C.
Boster Scientific Support
Answered: 2020-10-30