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Product Info Summary
| SKU: | EKC2265 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit
See all IFN-gamma R1/CD119 products
SKU/Catalog Number
EKC2265
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Interferon-gamma Receptor alpha (Phospho-Tyr457) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Interferon-gamma Receptor alpha (Phospho-Tyr457) protein expression profile in cells. The kit can be used for measuring the relative amounts of Interferon-gamma Receptor alpha (Phospho-Tyr457) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Interferon-gamma Receptor alpha (Phospho-Tyr457).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2265)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2265 is reactive to IFNGR1 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2265 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-IFNGR1 (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-IFNGR1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COS7 cells
Protein Target Info & Infographic
Gene/Protein Information For IFNGR1 (Source: Uniprot.Org, NCBI)
Gene Name
IFNGR1
Full Name
Interferon gamma receptor 1
Weight
54.405kDa
Superfamily
type II cytokine receptor family
Alternative Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2 IFNGR1 CD119, IFNGR, IMD27A, IMD27B interferon gamma receptor 1 interferon gamma receptor 1|AVP, type 2|CD119 antigen|CDw119|IFN-gamma receptor 1|IFN-gamma-R-alpha|IFN-gamma-R1|antiviral protein, type 2|immune interferon receptor 1|interferon-gamma receptor alpha chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNGR1, check out the IFNGR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNGR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit (EKC2265)
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Customer Q&As
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2 Customer Q&As for Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit
Question
I want to inquire about EKC2265, what is the approximate concentration range and the isotype of this antibody? What is the suitable secondary antibody for this primary antibody? Best Regards.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
EKC2265 Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit is sold at 100 ug per vial. We recommend to reconstitute this in 200 ul of sterile distilled water, so this will give a stock concentration of 0.5mg/ml. Regarding the isotype, this EKC2265 antibody is polyclonal, thus contains a mixture of all antibody isotypes but the main isotype of polyclonal is IgG. For the secondary antibodies we would suggest to use an anti-rabbit IgG.
Boster Scientific Support
Answered: 2020-04-21
Question
Could you please tell me if Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit only works using citrate buffer antigen retrieval pH6, or will EDTA pH8 will also work? Thanks. Lauren.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The validated records we have on Interferon-gamma Receptor alpha (Phospho-Tyr457) Colorimetric Cell-Based ELISA Kit only works on pH6 citrate buffer as a retrieval buffer for this antibody. But, it is possible that a different pH buffer will also work, however we do not have any experimental data on this.
Boster Scientific Support
Answered: 2020-04-20
