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Product Info Summary
| SKU: | EK1768 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse CXCL17 ELISA Kit PicoKine®
View all CXCL17/VCC-1 ELISA kits
SKU/Catalog Number
EK1768
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL17 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl17 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL17 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1768)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S23-L119
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1768 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1768 is reactive to Cxcl17 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1768 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL17/VCC-1
Chemokine (C-X-C motif) ligand 17 (CXCL17) is a small cytokine belonging to the CXC chemokine family that has been identified in humans and mice. It is mapped to 7; 7 A3. The protein encoded by this gene is a mucosal chemokine that attracts immature dendritic cells and blood monocytes to the lungs. The encoded protein also promotes tumorigenesis through an angiogenic activity. Finally, this protein exhibits strong antimicrobial activity against E. coli, S. aureus, Salmonella, P. aeruginosa, and C. albicans. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1768-CAP | Anti-Mouse Cxcl17 Pre-coated 96-well strip microplate | 1 |
| EK1768-ST | Mouse Cxcl17 Standard | 2 vials, 10 ng/tube |
| EK1768-DA | Mouse Cxcl17 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.004 | 0.144 | 0.237 | 0.365 | 0.674 | 0.973 | 1.584 | 2.166 |
Data Example Images
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Mouse CXCL17 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL17/VCC-1, Dilution ratio of 1:1, concentration in serum and plasma is around 1 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL17 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 281 | 647 | 4090 | 210 | 852 | 4469 |
| Standard deviation | 22.14 | 18.12 | 305.93 | 10.58 | 23.6 | 156.86 |
| CV(%) | 7.9% | 2.8% | 7.5% | 5.0% | 2.8% | 3.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL17/VCC-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 254 | 163 | 309 | 180 | 227 | 7.59 | 3.4% |
| Sample 2 | 313 | 396 | 389 | 313 | 353 | 24.27 | 6.9% |
| Sample 3 | 3010 | 4228 | 2934 | 3528 | 3425 | 160.29 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For Cxcl17 (Source: Uniprot.Org, NCBI)
Gene Name
Cxcl17
Full Name
C-X-C motif chemokine 17
Weight
13.627kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1 Cxcl17|VCC-1, Vcc1|chemokine (C-X-C motif) ligand 17|C-X-C motif chemokine 17|13.6-kDa protein|6-Cys CXCL17|VEGF co-regulated chemokine 1|VEGF coregulated chemokine 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cxcl17, check out the Cxcl17 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl17: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL17 ELISA Kit PicoKine® (EK1768)
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No publications found for EK1768
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Mouse CXCL17 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CXCL17 ELISA kit? I performed your CXCL17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CXCL17 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-09-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL17 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CXCL17 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-09-26
Question
Q: if the enzyme conjugated CXCL17 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL17 antigen?
R. Anderson
Verified customer
Asked: 2020-09-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-09-18
Question
Q: how to thaw whole blood sample for CXCL17 ELISA after freezing?
R. Robinson
Verified customer
Asked: 2020-06-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL17 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-06-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
A. Mangal
Verified customer
Asked: 2019-06-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-06-25
Question
Q: Can CXCL17 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
E. Lewis
Verified customer
Asked: 2016-02-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-02-10
