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Product Info Summary
| SKU: | EK7014 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Serum |
Product info
Product Name
Mouse Rat Testosterone ELISA Kit
SKU/Catalog Number
EK7014
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Testosterone. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Rat Testosterone ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7014)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.1 ng/ml
Assay Range
0.1-18 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7014 is reactive to in Mouse, Rat samples
Validated Sample Types
Serum
Application Guarantee
EK7014 is guaranteed for ELISA in Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Microwells coated with Goat Anti-Rabbit IgG | 12x8x1 Microwells |
| Standards | 6 vials ( ready to use) 0.5 ml |
| Rabbit Anti-Testosterone Reagent | 1 vial 7ml |
| Assay Diluent | 1 bottle (ready to use) 12ml |
| Enzyme Conjugate Conc. (20X) | 1 Vial 0.7ml |
| TMB Substrate | 1 bottle (ready to use) 12ml |
| Stop Solution | 1 bottle (ready to use) 12ml |
| 20X Wash Buffer | 1 bottle 25ml |
Standard Concentrations and example data
| Testosterone (ng/ml) | Absorbance (450nm) | |
|---|---|---|
| Std 1 | 0 | 2.38 |
| Std 2 | 0.1 | 1.75 |
| Std 3 | 0.5 | 1.02 |
| Std 4 | 2 | 0.59 |
| Std 5 | 6 | 0.34 |
| Std 6 | 18 | 0.17 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Protein Target Info & Infographic
Gene/Protein Information For (Source: Uniprot.org, NCBI)
Gene Name
Full Name
Weight
For more info on , check out the Infographic
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In this infographic, you will see the following information for : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Mouse Rat Testosterone ELISA Kit (EK7014)
Hello CJ!
EK7014 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Phospholipase C signaling activated by parathyroid hormone mediates the rapid osteoclastogenesis in the fracture healing of orchiectomized mice
Effect of Moxibustion on Testosterone Secretion and Apoptosis of Spermatogenic Cells in Aging Rats
Clusterin suppresses spermatogenic cell apoptosis to alleviate diabetes‐induced testicular damage by inhibiting autophagy via the PI3K/AKT/mTOR axis
4‐Methylcatechol inhibits cell growth and testosterone production in TM3 Leydig cells by reducing mitochondrial activity
Immunomodulatory effect of diallyl sulfide on experimentally-induced benign prostate hyperplasia via the suppression of CD4+T/IL-17 and TGF-β1/ERK pathways
Quercetin Modulates Aging Induced-Testicular Damage Via Increasing the Antioxidant Defense and the Immunolocalization of the Proliferating Cell Nuclear Antigen in Male Rats. Eman A. Al-Shahari,Abdelhalim A. Alkhazendar,Eman R. ElBealy,Abeer A. Alm-Eldeen
Species: Rat
EK7014 usage in article: APP:ELISA, SAMPLE:TESTES, DILUTION:NA
Wang E,Huang Y,Du Q,Sun Y. Alterations in reproductive parameters and gene expression in Balb/c mice testes after exposure to silver nanoparticles. Andrologia. 2020 Nov 9:e13841.doi:10.1111/and.13841.Epub ahead of print.PMID:33167059.
Species: Mouse
EK7014 usage in article: APP:ELISA, BLOOD, DILUTION:NA
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