Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex)

ELISA kit for Multiplex detection

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes: GMCSF, IFNα, IFNγ, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-6, IL-6R, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, TNFα. Attention: This product is from Quansys Bio and requires a special plate reader. Please make sure you have the proper equipment before ordering. For details, please contact [email protected].

Product Info Summary

Species Human
Sample Type Human Serum, EDTA/Heparin Plasma
Volume Required Per Well Min 25 µl
Assay Length 4.83 hours
Specificity All analytes < 1% intra-panel cross-reactivity
Assay Type Sandwich
Detection MethodChemiluminescent
Multiplex Format 96-well solid plate
Catalog NoMEK2007
Manufacturer Reference Number119733HU
AnalytesGMCSF, IFNα, IFNγ, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-6, IL-6R, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, TNFα

Product Overview

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours.

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

AnalytesCalibrator RangeUnitsLLD
GMCSF6,000-10.70pg/mL5.35
IFNα400-0.72pg/mL0.36
IFNγ99-0.18pg/mL0.09
IL-1β4,075-7.27pg/mL3.64
IL-1RA7,000-12.48pg/mL6.24
IL-264-0.11pg/mL0.06
IL-2Rα16,000-28.5pg/mL14.25
IL-6333-0.59pg/mL0.3
IL-6R1,250-2.23pg/mL1.11
IL-82,000-3.6pg/mL1.8
IL-102,680-4.78pg/mL2.39
IL-12p703,000-5.35pg/mL2.68
IL-13333-0.59pg/mL0.3
MCP-12,000-3.6pg/mL1.8
MIP-1α2,000-3.57pg/mL1.78
TNFα661-1.18pg/mL0.59

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.

Reagents include:

Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered solution with preservatives
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer
Plate Seals (3): Adhesive strips

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MEK2007
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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