Multiplex ELISA Kit For Mouse Cytokine - Inflammation (14-plex)

The Multiplex Mouse Cytokine – Inflammation (14-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 14 biomarkers or analytes. GMCSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα Cited in 1 publication(s).

Product Info Summary

Species: Mouse
Sample Type: Serum, Plasma
Volume Required per Well: Min 25 µl
Assay Length: 2.25 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1003
Manufacturer Reference Number: 110449MS
Analytes:GMCSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα

Product Overview

The Multiplex Mouse Cytokine – Inflammation (14-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 14 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 14 markers in the panel within 2.25 hours. The Multiplex Mouse Cytokine – Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Units Calibrator Range LLD Limit
GMCSF pg/ml 2000 - 2.74 2.30
IL-1α pg/ml 5000 - 6.86 4.20
IL-1β pg/ml 14000 - 19.20 12.41
IL-2 pg/ml 5000-6.86 3.44
IL-3 pg/ml 5000 - 6.86 3.18
IL-4 pg/ml 5000 - 6.86 2.50
IL-6 pg/ml 5000 - 6.86 2.90
IL-10 pg/ml 2000 - 2.75 3.00
IL-12p70 pg/ml 12000 - 16.46 12.20
IL-17 pg/ml 10000 - 13.72 6.20
MCP-1 pg/ml 3000 - 4.12 3.40
MIP-1α pg/ml 5000 - 6.86 0.61
RANTES pg/ml 3000 - 4.12 2.15
TNFα pg/ml 3000 - 4.12 3.40

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips

Product Images

MEK1003 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang Y, Tang J, Cai Z, Zhou K, Chang L, Bai Y, Ma Y. Prevotella Induces the Production of Th17 Cells in the Colon of Mice. J Immunol Res. 2020 Nov 1;2020:9607328. doi: 10.1155/2020/9607328. PMID: 33204736; PMCID: PMC7657696.
Species: Mouse
MEK1003 usage in article: APP:ELISA, SAMPLE:BMDC CELLS, DILUTION:NA

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3 Customer Q&As for Multiplex ELISA Kit For Mouse Cytokine - Inflammation (14-plex)

Question

Can MEK1003 be used for brain, liver and kidney tissues obtained from mice?

Verified customer

Asked: 2020-10-01

Answer

The Multiplex ELISA Kit For Mouse Cytokine - Inflammation (14-Plex) (MEK1003) is only validated for Cell Culture Supernates, Serum, and Plasma, though we've had a few customers send in homogenized brain tissue and homogenized lung tissue and obtain results. As long as it is homogenized, the kits should be able to provide results. If you are interested, we often offer to run a few samples to see if their samples will yield results.

Boster Scientific Support

Answered: 2020-10-01

Question

Which imager should be used for detection. Can they use “normal” chemiluminescence detector e.g for gel imaging?

Verified customer

Asked: 2019-08-29

Answer

A normal imager will not work with this kit. We do have demo packages where you can send samples to be tested or we can send a reader with a kit. Here are the demo options. https://www.bosterbio.com/multiplex-elisa-demo-options

Boster Scientific Support

Answered: 2019-09-03

Question

What is the recommended optimal dilution for MEK1003?

Verified customer

Asked: 2019-08-25

Answer

The Multiplex ELISA Kit For Mouse Cytokine - Inflammation (14-Plex) (MEK1003) is optimized at 1:2 dilution.

Boster Scientific Support

Answered: 2019-08-26

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MEK1003
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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