Custom Multiplex Assay Development

Q-Plex™ Technology offers quality imaging and data analysis for every application and budget

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Custom Multiplex ELISA Assays Built To Your Specifications

Top-of-the-line ISO 13485 certified assay development

With decades of experience developing sensitive and specific multiplex arrays for use in a wide variety of applications, our highly qualified team of assay developers, engineers, product managers, and specialized quality team can build a multiplex tool to meet your research needs.

Our highly efficient system allows us to keep costs low and assay accuracy high. Using our controlled manufacturing environment, we produce specialized multiplex ELISA kits with lyophilized reagents for long-term stability. We can also develop controls used for standard curve generation. Costs to develop a custom ELISA array will vary depending on the availability of reagents, the types of biomarkers desired, and the time involved to develop, optimize, and validate the ELISA array.

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Workflow Overview

Initial Feasibility

Request a feasibility assessment

Milestone I — Proof of Concept

Qualitative assays built

  • Reagent functionality and compatibility tested
  • Antibody and matched pair tested
  • Foreground to background determination
  • Intra-panel cross reactivity tested

Milestone II — Assay Optimization & Characterization

Calibrator formulation

  • Detection
  • Diluent optimization
  • Referencing standard
  • Protocol and incubation times
  • Characterization


  • Linearity/recovery
  • Sensitivity
  • Cross-reactivity
  • Backfit
  • Sample correlation
  • Interference

Milestone III — Iterative Design & Design Verification

Performance Verification

  • Precision
  • Linearity/recovery
  • Sensitivity
  • Cross-reactivity
  • Backfit
  • Drift
  • Edge effect
  • Sample correlation
  • Interference

Beta Kit Production & Testing

Pilot Production & QC Release

  • Create production documentation
  • Prepare plates and reagents
  • QC testing and release

Get started on your custom assay

Multiplex ELISA Solutions

In addition to Custom Assay Development, Boster provides Pre-made & Custom Printed Multiplex Kits, Imager + Kit Demo Package, and Sample Testing Service