CTLA4 (cytotoxic T-lymphocyte–associated antigen 4, aka CD152) is a transmembrane protein member of the immunoglobin superfamily. This protein receptor plays a key role in downregulating immune responses (e.g., inhibit T-cell activation).
Its expression relies on the stimulation of TCR and the binding between CD28 and B7 (B7-1/CD80 & B7-2/CD86), which both promote T-cell activation. Stimulating signals from TCR and CD28-B7 binding release CTLA4 from vesicles and translocates CTLA4 to the cell membrane where CTLA4 undergoes dimerization.
Since CTLA4 has a much higher affinity for B7, this competitive binding could prevent signals from CD28-B7 binding. CTLA4 may also inhibit T-cell activation through the dephosphorylation of TCR-signaling proteins or the interference with Akt signaling.
Click below to view & download the CTLA4 pathway map.
Boster takes great measures to ensure product quality and to provide our customers with comprehensive data upfront. Our antibodies are validated using WB, IHC, and flow cytometry against a panel of over 250 tissues and un-transfected cell lines to ensure high affinity and crystal-clear IHC stains. In addition, we also validate our antibodies in a quantitative fashion by testing them on known quantities of recombinant proteins so that you know what to expect (e.g. if there is 1ng, 2.5ng or 5ng of the target protein in the sample). Every product is covered by the Boster Quality Guarantee, click below to learn more.