Anti-SESTRIN2 SESN2 Antibody

Product Name

Anti-SESTRIN2 SESN2 Antibody

View all SESN2 Antibodies

SKU/Catalog Number

A02558

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SESTRIN2 SESN2 Antibody (Catalog # A02558). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SESTRIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SESTRIN2 SESN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02558)

Host

Rabbit

Contents

SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2. The immunogen is located within amino acids 220 - 270 of SESTRIN2.

Cross-reactivity

SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.

Reactive Species

A02558 is reactive to SESN2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.5 kDa

Background of SESN2

SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02558 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Recommend Dilution

SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SESN2

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