SDS-Polyacrylamide Gel Electrophoresis Kits and Reagents

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Introduction

Once your protein sample has been extracted, prepared, and quantified, it is time to perform gel electrophoresis. With proper preparation and execution, gel electrophoresis will cleanly separate the proteins by size, allowing you to measure not only your protein’s presence, but it’s relative size as well. Boster provides SDS-PAGE Gel Preparation kits and other high-quality reagents so you can get the most out of your samples.

Procedure

To prepare your sample for electrophoresis, mix the sample with Boster dual color protein loading buffer and denature the resulting mixture in a 100°C water bath. Once your sample is ready, fill your electrophoresis chamber with enough Boster electrophoresis buffer to cover the gel. Carefully pipette your sample into the wells, using enough volume to ensure consistent amounts of protein, and making sure not to poke the pipette tip into the gel. Connect your electrophoresis chamber to the power supply of your choice, and run the gel for enough time to adequately separate your protein sample.

After your gel has run for an appropriate amount of time, us one of Boster’s protein visualization reagents to verify the success and quality of your electrophoresis experiment.

For a detailed protocol for gel electrophoresis, see our Western blot handbook.

Tips

  1. For better results, optimize the gel concentration, SDS concentration, and run time and temperature prior to running the gel
  2. Ensure that each well has the same amount of protein before running the gel
  3. If you encounter streaked, distorted, or smile bands, check your electrophoresis parameters. See our troubleshooting guides for more information


Displaying 8 Items

    Commassie Blue Staining And Destaining Solution (AR0163)

    SKU AR0163
    Pack size1kit (for 10 assays to stain gels of 5 x 8.5cm)
    FormLiquid
    ApplicationsFor protein staining on polyacrylamide gels.

    Tris-Glycine SDS Running Buffer (10X) (AR1146-10)

    SKU AR1146-10
    Pack size 500ml (for 25 assays)
    Form Liquid
    Applications Running buffer for Tris-glycine gel electrophoresis

    PVP Antifade Mounting Medium (AR0036)

    • Specific References (4)
    SKU AR0036
    Pack size 10 mL (for 500 assays)
    ApplicationMounting tissue or cell slides for immunofluorescence
    Mounting slides for chromosome fluorescence
    Mounting slides for nucleic acid fluorescence in hybridization in situ

    Ponceau S Solution (AR0142-100)

    SKU AR0142-100
    Pack size 100 mL (1X)
    FormLiquid
    Applications Ponceau S solution has been used for membrane staining (Western blotting)

    Dual Color Protein Loading Buffer 4X (AR1142)

    • Specific References (2)
    Catalog numberUnit SizePrice (USD)
    AR11421mL X 6 (sufficient to make 24mL of protein solution)15
    AR1142-201mL X 20 (sufficient to make 80mL of protein solution)35

    SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112)

    • Specific References (15)
    • Q&As (2)
    Catalog numberUnit SizePrice (USD)
    AR11123mL10
    AR1112-2020mL45

    SDS-PAGE Protein Loading Buffer 2X (Reducing) (AR0131-20)

    • Specific References (4)
    SKU AR0131-20
    Pack size 20mL
    FormLiquid

    Tricine SDS Sample Buffer 2X (AR1143-20)

    • Specific References (1)
    SKU AR1143-20
    Pack size 20mL (20*1mL)
    FormLiquid

Displaying 8 Items