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Product Info Summary
| SKU: | LS386724 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of adhesion molecule with Ig-like domain 3 (AMIGO3) |
Product info
Product Name
AMIGO3 (NM_198722) Human Over-expression Lysate
SKU/Catalog Number
LS386724
Size
100 µg
Description
Transient overexpression lysate of adhesion molecule with Ig-like domain 3 (AMIGO3)
Storage & Handling
Cite This Product
AMIGO3 (NM_198722) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS386724)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AMIGO3 Human Over-expression Lysates NM_198722.
Western blot validation of overexpression lysate (LS386724) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AMIGO3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Amigo3 (Source: Uniprot.org, NCBI)
Gene Name
Amigo3
Full Name
Amphoterin-induced protein 3
Weight
55.565kDa
Superfamily
immunoglobulin superfamily
Alternative Names
adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552 Amigo3|adhesion molecule with Ig like domain 3|amphoterin-induced protein 3|AMIGO-3|alivin-3|amphoterin induced gene and ORF 3|transmembrane protein AMIGO3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Amigo3, check out the Amigo3 Infographic
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In this infographic, you will see the following information for Amigo3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AMIGO3 (NM_198722) Human Over-expression Lysate (LS386724)
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