Anti-14-3-3 theta/ tau YWHAQ Antibody

Boster Bio Anti-14-3-3 theta/ tau YWHAQ Antibody catalog # A03904. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03904
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-14-3-3 theta/ tau YWHAQ Antibody

See all YWHAQ products

SKU/Catalog Number

A03904

Size

100μl

Form

Liquid

Description

Boster Bio Anti-14-3-3 theta/ tau YWHAQ Antibody catalog # A03904. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-14-3-3 theta/ tau YWHAQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03904)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Reactive Species

A03904 is reactive to YWHAQ in Human, Mouse, Rat

Applications

A03904 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For YWHAQ (Source: Uniprot.Org, NCBI)

Uniprot ID

P27348

Gene ID

10971

Gene Name

YWHAQ

Full Name

14-3-3 protein theta

Weight

27.764kDa

Superfamily

14-3-3 family

Alternative Names

14-3-3 protein tau; 14-3-3 protein T-cell; 14-3-3 protein theta; 1433; 14-3-3; 14-3-3,1C5; HS1; Protein HS1; protein tau; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide YWHAQ 14-3-3, 1C5, HS1 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta 14-3-3 protein theta|14-3-3 protein T-cell|14-3-3 protein tau|14-3-3 theta|protein, theta|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on YWHAQ, check out the YWHAQ Infographic

YWHAQ infographic

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In this infographic you will see the following information for YWHAQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Anti-14-3-3 theta/ tau YWHAQ Antibody

Question

We are currently using anti-14-3-3 theta/ tau antibody A03904 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The anti-14-3-3 theta/ tau antibody (A03904) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-04

Question

Our lab were happy with the WB result of your anti-14-3-3 theta/ tau antibody. However we have been able to see positive staining in skin uterus cytoplasm. note=in neurons using this antibody. Is that expected? Could you tell me where is YWHAQ supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

According to literature, skin uterus does express YWHAQ. Generally YWHAQ expresses in cytoplasm. note=in neurons, axonally. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305

Boster Scientific Support

Answered: 2020-01-31

Question

We have observed staining in human leukemic t-cell. What should we do? Is anti-14-3-3 theta/ tau antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

From what I have seen in literature leukemic t-cell does express YWHAQ. From what I have seen in Uniprot.org, YWHAQ is expressed in substantia nigra, t-cell, keratinocyte, placenta, skin uterus, platelet, b-cell lymphoma hepatoma, brain, lymphoblast, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305

Boster Scientific Support

Answered: 2019-09-05

Size

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A03904
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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