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Product Info Summary
| SKU: | A03904 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-14-3-3 theta/ tau YWHAQ Antibody
SKU/Catalog Number
A03904
Size
100μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 theta/ tau YWHAQ Antibody catalog # A03904. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 theta/ tau YWHAQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03904)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau. AA range:196-245
Reactive Species
A03904 is reactive to YWHAQ in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
27.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03904 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of Hela cells using 14-3-3 θ/τ Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of MCF7 cell. 1, primary Antibody was diluted at 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500 (room temperature, 50min).
Specific Publications For Anti-14-3-3 theta/ tau YWHAQ Antibody (A03904)
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Customer Q&As
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3 Customer Q&As for Anti-14-3-3 theta/ tau YWHAQ Antibody
Question
We are currently using anti-14-3-3 theta/ tau antibody A03904 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-14-3-3 theta/ tau antibody (A03904) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Our lab were happy with the WB result of your anti-14-3-3 theta/ tau antibody. However we have been able to see positive staining in skin uterus cytoplasm. note=in neurons using this antibody. Is that expected? Could you tell me where is YWHAQ supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to literature, skin uterus does express YWHAQ. Generally YWHAQ expresses in cytoplasm. note=in neurons, axonally. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305
Boster Scientific Support
Answered: 2020-01-31
Question
We have observed staining in human leukemic t-cell. What should we do? Is anti-14-3-3 theta/ tau antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
From what I have seen in literature leukemic t-cell does express YWHAQ. From what I have seen in Uniprot.org, YWHAQ is expressed in substantia nigra, t-cell, keratinocyte, placenta, skin uterus, platelet, b-cell lymphoma hepatoma, brain, lymphoblast, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305
Boster Scientific Support
Answered: 2019-09-05
