Click image to see more details
Product Info Summary
| SKU: | M01600 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ATF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01600
BM4696 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATF1 Rabbit Monoclonal Antibody catalog # M01600. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01600)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HBF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATF1
Reactive Species
M01600 is reactive to ATF1 in Human
Observed Molecular Weight
36 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01600 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATF1 expression in Hela cell lysate.
Specific Publications For Anti-ATF1 Rabbit Monoclonal Antibody (M01600)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ATF1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ATF1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-ATF1 Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ATF1 Rabbit Monoclonal antibody M01600. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this M01600 anti-ATF1 Rabbit Monoclonal antibody reactive to the isotypes of ATF1?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The immunogen of M01600 anti-ATF1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ATF1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-17
Question
Would anti-ATF1 Rabbit Monoclonal antibody M01600 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of leukemic t-cell, ATF1 is highly expressed in leukemic t-cell. So, it is likely that anti-ATF1 Rabbit Monoclonal antibody M01600 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-ATF1 Rabbit Monoclonal antibody M01600 available?
R. Wu
Verified customer
Asked: 2019-08-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATF1 Rabbit Monoclonal antibody M01600 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-08
Question
I was wanting to use your anti-ATF1 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
It shows on the product datasheet, M01600 anti-ATF1 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
Does M01600 anti-ATF1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Carter
Verified customer
Asked: 2018-05-02
Answer
As indicated on the product datasheet, M01600 anti-ATF1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-02
Question
Would anti-ATF1 Rabbit Monoclonal antibody M01600 work on horse WB with cervix carcinoma erythroleukemia?
S. Jha
Verified customer
Asked: 2014-12-05
Answer
Our lab technicians have not validated anti-ATF1 Rabbit Monoclonal antibody M01600 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ATF1 Rabbit Monoclonal antibody M01600 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-05
