Anti-ATF1 Rabbit Monoclonal Antibody

ATF1 antibody

Boster Bio Anti-ATF1 Rabbit Monoclonal Antibody catalog # M01600. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M01600
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, ICC, WB

Product Name

Anti-ATF1 Rabbit Monoclonal Antibody

View all ATF1 Antibodies

SKU/Catalog Number

M01600

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ATF1 Rabbit Monoclonal Antibody catalog # M01600. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01600)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HBF-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ATF1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01600 is reactive to ATF1 in Human

Applications

M01600 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

92 kDa

Calculated molecular weight

29.233kDa

Background of ATF1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ATF1 (Source: Uniprot.org, NCBI)

Gene Name

ATF1

Full Name

Cyclic AMP-dependent transcription factor ATF-1

Weight

29.233kDa

Superfamily

bZIP family

Alternative Names

activating transcription factor 1FUS/ATF-1; ATF1; cAMP-dependent transcription factor ATF-1; EWS-ATF1; Protein TREB36; TREB36; TREB36cyclic AMP-dependent transcription factor ATF-1 ATF1 EWS-ATF1, FUS/ATF-1, TREB36 activating transcription factor 1 cyclic AMP-dependent transcription factor ATF-1|cAMP-dependent transcription factor ATF-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATF1, check out the ATF1 Infographic

ATF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ATF1 Rabbit Monoclonal Antibody

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ATF1 Rabbit Monoclonal antibody M01600. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-04

Question

Is this M01600 anti-ATF1 Rabbit Monoclonal antibody reactive to the isotypes of ATF1?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

The immunogen of M01600 anti-ATF1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ATF1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-17

Question

Would anti-ATF1 Rabbit Monoclonal antibody M01600 work for IF with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

According to the expression profile of leukemic t-cell, ATF1 is highly expressed in leukemic t-cell. So, it is likely that anti-ATF1 Rabbit Monoclonal antibody M01600 will work for IF with leukemic t-cell.

Boster Scientific Support

Answered: 2019-10-24

Question

Do you have a BSA free version of anti-ATF1 Rabbit Monoclonal antibody M01600 available?

R. Wu

Verified customer

Asked: 2019-08-08

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATF1 Rabbit Monoclonal antibody M01600 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-08

Question

I was wanting to use your anti-ATF1 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It shows on the product datasheet, M01600 anti-ATF1 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

Does M01600 anti-ATF1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Carter

Verified customer

Asked: 2018-05-02

Answer

As indicated on the product datasheet, M01600 anti-ATF1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-02

Question

Would anti-ATF1 Rabbit Monoclonal antibody M01600 work on horse WB with cervix carcinoma erythroleukemia?

S. Jha

Verified customer

Asked: 2014-12-05

Answer

Our lab technicians have not validated anti-ATF1 Rabbit Monoclonal antibody M01600 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ATF1 Rabbit Monoclonal antibody M01600 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-12-05

Order DetailsPrice
M01600

100μL

$370
M01600-30ul

30μL sample

$99
M01600-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M01600
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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