Product Info Summary
SKU: | A02008 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ACAT1 (K266) Antibody
SKU/Catalog Number
A02008
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ACAT1 (K266) Antibody catalog # A02008. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACAT1 (K266) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02008)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Immunogen
Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02008 is reactive to ACAT1 in Human, Mouse, Rat
Applications
A02008 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
45.2kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilution
Lane1:HEK293T whole cell lysate(20ug)
Lane2:HepG2 whole cell lysate(20ug)
Lane3:The Lung tissue lysate of Rat(20ug)
Lane4:The Lung tissue lysate of Mouse(10ug)
Click image to see more details
Western blot (WB) analysis of ACAT1 (K266) pAb at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:SP2/0 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)
Lane5:PC12 whole cell lysate(40ug)
Lane6:NIH-3 whole cell lysate(40ug)
Click image to see more details
Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Click image to see more details
Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Click image to see more details
Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-b
Protein Target Info & Infographic
Gene/Protein Information For ACAT1 (Source: Uniprot.org, NCBI)
Gene Name
ACAT1
Full Name
Acetyl-CoA acetyltransferase, mitochondrial
Weight
45.2kDa
Superfamily
thiolase-like superfamily
Alternative Names
ACAT; ACAT1; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; EC 2.3.1; EC 2.3.1.9; MAT; mitochondrial acetoacetyl-CoA thiolase; T2; THIL ACAT1 ACAT, MAT, T2, THIL acetyl-CoA acetyltransferase 1 acetyl-CoA acetyltransferase, mitochondrial|acetoacetyl Coenzyme A thiolase|acetoacetyl-CoA thiolase|acetyl-Coenzyme A acetyltransferase 1|mitochondrial acetoacetyl-CoA thiolase|testicular tissue protein Li 198
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACAT1, check out the ACAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACAT1 (K266) Antibody (A02008)
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No publications found for A02008
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