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Product Info Summary
| SKU: | A03335 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DRA SLC26A3 Antibody
SKU/Catalog Number
A03335
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DRA SLC26A3 Antibody catalog # A03335. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DRA SLC26A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03335)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human DRA.
Reactive Species
A03335 is reactive to SLC26A3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
84.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03335 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Phosphocholine decreases fluid absorption by increasing cAMP levels via the activation of adenylyl cyclase. The levels of cAMP a and cGMP b in the colon, and the activity of adenylyl cyclase c and guanylyl cyclase d ; data were analyzed with two-tailed Student’s t-test and presented as mean ± SEM. n = 6. * P < 0.05. e Western blots showing the protein expression of SLC9A3, SLC5A1, SLC26A3, and GAPDH in the colon; cAMP content f , adenylyl cyclase activity g , cGMP content h , and guanylyl cyclase activity i in the primary colonocytes; j Western blots showing the protein expression of SLC9A3, SLC5A1, SLC26A3 and GAPDH in the primary colonocytes; Relative level of Na + k and Cl - l in the culture medium. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01.
Index in PubMed under a CC BY license. PMID: 37666845
Specific Publications For Anti-DRA SLC26A3 Antibody (A03335)
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6 Customer Q&As for Anti-DRA SLC26A3 Antibody
Question
We are currently using anti-DRA antibody A03335 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-DRA antibody (A03335) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-DRA antibody A03335. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this A03335 anti-DRA antibody reactive to the isotypes of SLC26A3?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The immunogen of A03335 anti-DRA antibody is Synthesized peptide derived from the C-terminal region of human DRA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-28
Question
Will A03335 anti-DRA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
As indicated on the product datasheet, A03335 anti-DRA antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-29
Question
See attached the WB image, lot number and protocol we used for lung using anti-DRA antibody A03335. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-09
Question
Is there a BSA free version of anti-DRA antibody A03335 available?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DRA antibody A03335 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-09
