Human MMP-8 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8. 96wells/kit, with removable strips. Cited in 3 publication(s).
Human MMP-8 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin) and saliva.|
|Sample Volume:||100μl per well|
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Human MMP-8 ELISA Kit PicoKine™
See all MMP8 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human MMP8 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin) and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP8 tissue specificity: Neutrophils.|
|Cite This Product||Human MMP-8 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0464)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin) and saliva.|
|Immunogen||Expression system for standard: NSO; Immunogen sequence: F21-G467|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Neutrophil collagenase|
|Subcellular Localization||Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Can degrade fibrillar type I, II, and III collagens.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human MMP-8 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0464-CAP||96-well plate precoated with anti-Human MMP8 antibody||1|
|EK0464-ST||lyophilized recombinant Human MMP8 standard||10ng/tube|
|EK0464-DA||biotinylated anti-Human MMP8 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0464 Human MMP-8 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human MMP-8 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human MMP-8 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 3
|Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...||PubMed ID 25655502|
|Kumar Y, Jain V, Chauhan SS, Bharate V, Koli D, Kumar M. J Prosthet Dent. 2017 Mar 23. pii: S0022-3913(16)30693-X. doi: 10.1016/j.prosdent.2016.11.017. Influence of different forms and materials (zirconia or titanium) of abutments in periimplant s...||PubMed ID 28343676|
|Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test||PubMed ID 26644719|
Order Product (EK0464)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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