Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™

Human MMP-8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0464
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and saliva.

Product Name

Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™

See all MMP-8 products

SKU/Catalog Number

EK0464

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0464)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: F21-G467

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0464 is reactive to MMP8 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and saliva.

Application Guarantee

EK0464 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0464-CAP Anti-Human MMP8 Pre-coated 96-well strip microplate 1
EK0464-ST Human MMP8 Standard 2 vials, 10 ng/tube
EK0464-DA Human MMP8 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0830.3340.4860.7401.1811.4681.6731.930

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-8, Dilution ratio of 1:30, concentration in serum is around 40ng/ml.

Some articles we found to cite concentrations of MMP-8 in samples: 17546376 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2511516501025815385119
Standard deviation13.05101.57360.7218.31109.19404.4
CV (%)5.2%6.7%7.2%7.1%7.1%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-8 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 125124727527326112.594.8%
Sample 21516131213471465141083.415.9%
Sample 350105134482147024916166.713.3%
*number of samples for each test n=16.

Gene/Protein Information For MMP8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22894

Gene ID

4317

Gene Name

MMP8

Full Name

Neutrophil collagenase

Weight

53.412kDa

Superfamily

peptidase M10A family

Alternative Names

CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL MMP8 CLG1, HNC, MMP-8, PMNL-CL matrix metallopeptidase 8 neutrophil collagenase|PMN leukocyte collagenase|PMNL collagenase|collagenase 2|matrix metalloproteinase 8 (neutrophil collagenase)|matrix metalloproteinase-8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP8, check out the MMP8 Infographic

MMP8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0464 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tu CC, Wang SH, Hu CY, Chen MH, Hsieh O, Chang PC. Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial. J Formos Med Assoc. 2020 Nov 5:S0929-6646(20)30503-
Species: Human
EK0464 usage in article: APP:ELISA, SAMPLE:GINGIVAL CERVICULAR FLUID, DILUTION:NA

Expression of matrix metalloproteinase-8 and matrix metalloproteinase-13 in mast cells of human periapical lesions

Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test

Kumar Y, Jain V, Chauhan SS, Bharate V, Koli D, Kumar M. J Prosthet Dent. 2017 Mar 23. pii: S0022-3913(16)30693-X. doi: 10.1016/j.prosdent.2016.11.017. Influence of different forms and materials (zirconia or titanium) of abutments in periimplant s...

Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...

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10 Customer Q&As for Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™

Question

Q: can I use EDTA plasma as samples in Human MMP-8 Picokine® ELISA Kit (Catalog # EK0464)?

Verified Customer

Verified customer

Asked: 2020-05-30

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MMP-8 causing disruption of its protein structure. MMP-8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MMP-8 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-05-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

M. Dhar

Verified customer

Asked: 2019-10-07

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-10-07

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-25

Question

How many reactions are included in one EK0464 kit?

Verified customer

Asked: 2019-03-28

Answer

There are 12 strips of 8 wells each in the Human MMP-8 ELISA Kit PicoKine™ (EK0464), all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit.

Boster Scientific Support

Answered: 2019-03-28

Question

Are the antibodies suitable for Western Blot application?

Verified customer

Asked: 2019-03-28

Answer

The antibody in the Human MMP-8 ELISA Kit PicoKine™ (EK0464) and all our ELISA kits are not validated for Western Blot usage.

Boster Scientific Support

Answered: 2019-03-28

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-10-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-28

Question

Q: how to analyze ELISA data? I have obtained MMP-8 level in saliva.

H. Lopez

Verified customer

Asked: 2018-02-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-02-09

Question

Q: if the enzyme conjugated MMP-8 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-8 antigen?

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-10-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. Singh

Verified customer

Asked: 2015-08-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2015-08-19

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of MMP-8 in Human saliva? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

N. Jones

Verified customer

Asked: 2014-01-27

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-8 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-01-27

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