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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin) and saliva.|
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Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP-8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0464)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: F21-G467
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0464 is reactive to MMP8 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and saliva.
EK0464 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0464-CAP||Anti-Human MMP8 Pre-coated 96-well strip microplate||1|
|EK0464-ST||Human MMP8 Standard||2 vials, 10 ng/tube|
|EK0464-DA||Human MMP8 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP-8 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-8, Dilution ratio of 1:30, concentration in serum is around 40ng/ml.
Some articles we found to cite concentrations of MMP-8 in samples: 17546376 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-8 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP8 (Source: Uniprot.Org, NCBI)
peptidase M10A family
CLG1HNC; Collagenase 2; EC 3.4.24; EC 184.108.40.206; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL MMP8 CLG1, HNC, MMP-8, PMNL-CL matrix metallopeptidase 8 neutrophil collagenase|PMN leukocyte collagenase|PMNL collagenase|collagenase 2|matrix metalloproteinase 8 (neutrophil collagenase)|matrix metalloproteinase-8*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP8, check out the MMP8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0464 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tu CC, Wang SH, Hu CY, Chen MH, Hsieh O, Chang PC. Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial. J Formos Med Assoc. 2020 Nov 5:S0929-6646(20)30503-
EK0464 usage in article: APP:ELISA, SAMPLE:GINGIVAL CERVICULAR FLUID, DILUTION:NA
Expression of matrix metalloproteinase-8 and matrix metalloproteinase-13 in mast cells of human periapical lesions
Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test
Kumar Y, Jain V, Chauhan SS, Bharate V, Koli D, Kumar M. J Prosthet Dent. 2017 Mar 23. pii: S0022-3913(16)30693-X. doi: 10.1016/j.prosdent.2016.11.017. Influence of different forms and materials (zirconia or titanium) of abutments in periimplant s...
Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...
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