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Human MMP-8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva.. Sensitivity: 10pg/ml. Cited in 5 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum, plasma (heparin) and saliva.|
|Sample Volume:||100μl per well|
|Assay Range:||156 pg/ml - 10,000 pg/ml|
|Product Name||Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™
See more MMP-8 products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human MMP-8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva.. Sensitivity: 10pg/ml.|
|Cite This Product||Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0464)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum, plasma (heparin) and saliva.|
|Assay Range||156 pg/ml - 10,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: F21-G467|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||4317|
|Species Of This Entry||Human|
|Protein Name||Neutrophil collagenase|
|Superfamily||peptidase M10A family|
|Alternative Names||MMP-8|CLG1HNC; Collagenase 2; EC 3.4.24; EC 184.108.40.206; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for MMP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0464-CAP||Anti-Human MMP8 Pre-coated 96-well strip microplate||1|
|EK0464-ST||Human MMP8 Standard||2 vials, 10 ng/tube|
|EK0464-DA||Human MMP8 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0464 Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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|Tu CC, Wang SH, Hu CY, Chen MH, Hsieh O, Chang PC. Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial. J Formos Med Assoc. 2020 Nov 5:S0929-6646(20)30503-
EK0464 usage in article: APP:ELISA, SAMPLE:GINGIVAL CERVICULAR FLUID, DILUTION:NA
|PubMed ID 33162294|
|Expression of matrix metalloproteinase-8 and matrix metalloproteinase-13 in mast cells of human periapical lesions||PubMed ID 31938366|
|Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test||PubMed ID 26644719|
|Kumar Y, Jain V, Chauhan SS, Bharate V, Koli D, Kumar M. J Prosthet Dent. 2017 Mar 23. pii: S0022-3913(16)30693-X. doi: 10.1016/j.prosdent.2016.11.017. Influence of different forms and materials (zirconia or titanium) of abutments in periimplant s...||PubMed ID 28343676|
|Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...||PubMed ID 25655502|
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Comprehensive info on MMP8, beautifully presented.Open Infographic
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