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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine.|
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Human Adiponectin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Adiponectin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0595)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E19-N244
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0595 is reactive to ADIPOQ in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EK0595 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0595-CAP||Anti-Human ADIPOQ Pre-coated 96-well strip microplate||1|
|EK0595-ST||Human ADIPOQ Standard||2 vials, 100 ng/tube|
|EK0595-DA||Human ADIPOQ Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Adiponectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Adiponectin/Acrp30, Dilution ratio of 1:5000, concentration in serum is around 90ug/ml.
Some articles we found to cite concentrations of Adiponectin/Acrp30 in samples: 25528313, 25454366 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Adiponectin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For ADIPOQ (Source: Uniprot.Org, NCBI)
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ADIPOQ, check out the ADIPOQ Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0595 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Foula WH,Emara RH,Eldeeb MK,Mokhtar SA,El-Sahn FA. Effect of a weight loss program on serum adiponectin and insulin resistance among overweight and obese premenopausal females. J Egypt Public Health Assoc.2020 Dec 1;95(1):32.doi: 10.1186/s42506-020-00060-
EK0595 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Adiponectin protects against uric acid‑induced renal tubular epithelial inflammatory responses via the AdipoR1/AMPK signaling pathway
The emerging role of leptin, Adiponectin and Visfatin in Ischemic/Hemorrhagic stroke
The Effects of Adjuvant Endocrine Treatment on Serum Leptin, Serum Adiponectin and Body Composition in Patients with Breast Cancer: The Izmir Oncology Group (IZOG) Study
Relation of serum irisin level with metabolic and antropometric parameters in obese children.
Oxidative stress and adipokine levels were significantly correlated in diabetic patients with hyperglycemic crises.
The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity
The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical trial
Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway
Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals
Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...
Mohammadi T, Paknahad Z. Clin Nutr Res. 2017 Oct;6(4):267-276. doi: 10.7762/cnr.2017.6.4.267. Epub 2017 Oct 27. Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study
Chen S, Li J, Zhang Z, Li W, Sun Y, Zhang Q, Feng X, Zhu W. Can J Physiol Pharmacol. 2012 Feb;90(2):237-42. Doi: 10.1139/Y11-123. Epub 2012 Feb 6. Effects Of Resveratrol On The Amelioration Of Insulin Resistance In Kkay Mice.
Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).
Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...
Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...
Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.
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