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Product Info Summary
| SKU: | EK0595 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Adiponectin ELISA Kit PicoKine®
SKU/Catalog Number
EK0595
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Adiponectin ELISA Kit PicoKine® (96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Adiponectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0595)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E19-N244
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<60 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK0595 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0595 is reactive to ADIPOQ in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0595 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ADIPOQ
Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0595-CAP | Anti-Human ADIPOQ Pre-coated 96-well strip microplate | 1 |
| EK0595-ST | Human ADIPOQ Standard | 2 vials, 100 ng/tube |
| EK0595-DA | Human ADIPOQ Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.056 | 0.105 | 0.148 | 0.247 | 0.382 | 0.644 | 1.103 | 1.878 |
Data Example Images
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Human Adiponectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ADIPOQ, Dilution ratio of 1:1000, concentration in serum and plasma is around 20 ug/ml..
Some articles we found to cite concentrations of ADIPOQ in samples: 25528313, 25454366 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Adiponectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3726 | 12058 | 49927 | 3646 | 11478 | 49590 |
| Standard deviation | 182.57 | 940.52 | 2346.56 | 236.99 | 1033.02 | 2578.68 |
| CV (%) | 4.9% | 7.8% | 4.7% | 6.5% | 9% | 5.2% |
Specific Publications For Human Adiponectin ELISA Kit PicoKine® (EK0595)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human Adiponectin ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human Adiponectin Picokine® ELISA Kit (Catalog # EK0595)?
Verified Customer
Verified customer
Asked: 2020-08-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Adiponectin causing disruption of its protein structure. Adiponectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Adiponectin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-13
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
K. White
Verified customer
Asked: 2020-06-18
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-18
Question
Q: What is the optimal O.D. value for Adiponectin ELISA kit? I used your Adiponectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Adiponectin even though the O.D. values are not very high?
Z. Williams
Verified customer
Asked: 2020-02-16
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Adiponectin ELISA kit to have sensitivity of 60pg/ml, that means the minimum amount of Adiponectin that can be declared/interpreted as positive by the above standard is 60pg/ml.
Boster Scientific Support
Answered: 2020-02-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. White
Verified customer
Asked: 2018-09-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-26
Question
What is the reference range for this kit in human serum?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
The reference range for this kit in human serum is 1000-40000ng/mL.
Boster Scientific Support
Answered: 2017-11-09
Question
Q: if the enzyme conjugated Adiponectin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Adiponectin antigen?
H. Liu
Verified customer
Asked: 2016-07-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-07-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
G. Green
Verified customer
Asked: 2015-09-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-09-24
Question
Greetings!! We have a question regarding your products EK0595, EK0437 Actually, this question can suit for all your ELISA kits Do you have a standard for the value of the R2 value? We have a customer that used your product and the R2 results can go from 0.996~0.999 So the customer asked us if there is a standard for a value so if the customer's R2 reaches that value, the results will be convinced
Verified Customer
Verified customer
Asked: 2013-03-18
Answer
The kinetic rate of the reaction between the protein and the antibody is different, which results in a different way of fitting. We can not say that other fitting methods are good or not good and we can only say that we should choose the equation that is most suitable for our own experimental results, generally based on the degree of fitness, taking into account the trend of the curve. For the kits from the same batch, we recommend the customer to generate a specific standard curve for each box first and then calculate the data. The data amoung different kits from the same batch would be different slightly due to influential factors such as time difference, temperature difference, operation difference. It is ok to use a different equation to generate the standard curves for different plates to compare them, but the R value should be considered.
Boster Scientific Support
Answered: 2013-03-18
