Human Adiponectin ELISA Kit PicoKine™

Human Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. Cited in 19 publication(s).

Product Info Summary

SKU: EK0595
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human Adiponectin ELISA Kit PicoKine™

See all Adiponectin/Acrp30 products

SKU/Catalog Number

EK0595

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Adiponectin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0595)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N244

Sensitivity

<60 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0595 is reactive to ADIPOQ in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0595 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0595-CAP Anti-Human ADIPOQ Pre-coated 96-well strip microplate 1
EK0595-ST Human ADIPOQ Standard 2 vials, 100 ng/tube
EK0595-DA Human ADIPOQ Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0140.1050.1700.3260.5960.9791.5071.967

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Adiponectin/Acrp30, Dilution ratio of 1:5000, concentration in serum is around 90ug/ml.

Some articles we found to cite concentrations of Adiponectin/Acrp30 in samples: 25528313, 25454366 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Adiponectin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3726120584992736461147849590
Standard deviation182.57940.522346.56236.991033.022578.68
CV (%)4.9%7.8%4.7%6.5%9%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13726381038493865381253.791.4%
Sample 21205812135116221035711543712.136.1%
Sample 349927481965275450855504331645.093.2%
*number of samples for each test n=16.

Gene/Protein Information For ADIPOQ (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15848

Gene ID

9370

Gene Name

ADIPOQ

Full Name

Adiponectin

Weight

26.414kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADIPOQ, check out the ADIPOQ Infographic

ADIPOQ infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0595 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Foula WH,Emara RH,Eldeeb MK,Mokhtar SA,El-Sahn FA. Effect of a weight loss program on serum adiponectin and insulin resistance among overweight and obese premenopausal females. J Egypt Public Health Assoc.2020 Dec 1;95(1):32.doi: 10.1186/s42506-020-00060-
Species: Human
EK0595 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Adiponectin protects against uric acid‑induced renal tubular epithelial inflammatory responses via the AdipoR1/AMPK signaling pathway

The emerging role of leptin, Adiponectin and Visfatin in Ischemic/Hemorrhagic stroke

The Effects of Adjuvant Endocrine Treatment on Serum Leptin, Serum Adiponectin and Body Composition in Patients with Breast Cancer: The Izmir Oncology Group (IZOG) Study

Relation of serum irisin level with metabolic and antropometric parameters in obese children.

Oxidative stress and adipokine levels were significantly correlated in diabetic patients with hyperglycemic crises.

The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity

The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical trial

Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals

Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...

Mohammadi T, Paknahad Z. Clin Nutr Res. 2017 Oct;6(4):267-276. doi: 10.7762/cnr.2017.6.4.267. Epub 2017 Oct 27. Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study

Chen S, Li J, Zhang Z, Li W, Sun Y, Zhang Q, Feng X, Zhu W. Can J Physiol Pharmacol. 2012 Feb;90(2):237-42. Doi: 10.1139/Y11-123. Epub 2012 Feb 6. Effects Of Resveratrol On The Amelioration Of Insulin Resistance In Kkay Mice.

Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).

Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...

Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...

Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...

Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.

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8 Customer Q&As for Human Adiponectin ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human Adiponectin Picokine® ELISA Kit (Catalog # EK0595)?

Verified Customer

Verified customer

Asked: 2020-08-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Adiponectin causing disruption of its protein structure. Adiponectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Adiponectin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-13

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

K. White

Verified customer

Asked: 2020-06-18

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-18

Question

Q: What is the optimal O.D. value for Adiponectin ELISA kit? I used your Adiponectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Adiponectin even though the O.D. values are not very high?

Z. Williams

Verified customer

Asked: 2020-02-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Adiponectin ELISA kit to have sensitivity of 60pg/ml, that means the minimum amount of Adiponectin that can be declared/interpreted as positive by the above standard is 60pg/ml.

Boster Scientific Support

Answered: 2020-02-16

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. White

Verified customer

Asked: 2018-09-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-26

Question

What is the reference range for this kit in human serum?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

The reference range for this kit in human serum is 1000-40000ng/mL.

Boster Scientific Support

Answered: 2017-11-09

Question

Q: if the enzyme conjugated Adiponectin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Adiponectin antigen?

H. Liu

Verified customer

Asked: 2016-07-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-07-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

G. Green

Verified customer

Asked: 2015-09-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-09-24

Question

Greetings!! We have a question regarding your products EK0595, EK0437 Actually, this question can suit for all your ELISA kits Do you have a standard for the value of the R2 value? We have a customer that used your product and the R2 results can go from 0.996~0.999 So the customer asked us if there is a standard for a value so if the customer's R2 reaches that value, the results will be convinced

Verified Customer

Verified customer

Asked: 2013-03-18

Answer

The kinetic rate of the reaction between the protein and the antibody is different, which results in a different way of fitting. We can not say that other fitting methods are good or not good and we can only say that we should choose the equation that is most suitable for our own experimental results, generally based on the degree of fitness, taking into account the trend of the curve. For the kits from the same batch, we recommend the customer to generate a specific standard curve for each box first and then calculate the data. The data amoung different kits from the same batch would be different slightly due to influential factors such as time difference, temperature difference, operation difference. It is ok to use a different equation to generate the standard curves for different plates to compare them, but the R value should be considered.

Boster Scientific Support

Answered: 2013-03-18

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