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Product Info Summary
| SKU: | A14001-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ZBED3 Antibody
SKU/Catalog Number
A14001-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBED3 Antibody (Catalog # A14001-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBED3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14001-1)
Host
Rabbit
Contents
ZBED3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBED3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ZBED3. The immunogen is located within the first 50 amino acids of ZBED3.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
Reactive Species
A14001-1 is reactive to ZBED3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.1 kDa
Background of ZBED3
Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14001-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBED3 in A-20 cell lysate with ZBED3 antibody at 1 μg/ml.
Specific Publications For Anti-ZBED3 Antibody (A14001-1)
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