Mouse Adiponectin ELISA Kit PicoKine™

Mouse Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 19 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0596
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Mouse Adiponectin ELISA Kit PicoKine™

See all Adiponectin/Acrp30 products

SKU/Catalog Number

EK0596

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Adiponectin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0596)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-N244

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0596 is reactive to Adipoq in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0596 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0596-CAP Anti-Mouse Adipoq Pre-coated 96-well strip microplate 1
EK0596-ST Mouse Adipoq Standard 2 vials, 20 ng/tube
EK0596-DA Mouse Adipoq Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0000.2980.5410.8971.4741.9462.1832.254

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Adiponectin/Acrp30, Dilution ratio of 1:2000, concentration in serum is 4.8-6.1ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Adiponectin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5672994757251231217518
Standard deviation37.98179.64318.0237.75240.31330.79
CV (%)6.7%6%4.2%7.1%7.7%4.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 156760362857159224.914.2%
Sample 22994286728452816288067.972.3%
Sample 375727510816774137665295.033.8%
*number of samples for each test n=16.

Gene/Protein Information For Adipoq (Source: Uniprot.Org, NCBI)

Uniprot ID

Q60994

Gene ID

11450

Gene Name

Adipoq

Full Name

Adiponectin

Weight

26.809kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein Adipoq|30kDa, APN, Acd, Acdc, Acr, Acrp30, Ad, Adid, GBP2, GBP28, a, adip, adipo, ap, apM1|adiponectin, C1Q and collagen domain containing|adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement related protein|adipocyte complement-related 30 kDa protein|adipocyte, C1Q and collagen domain containing|adipocyte, C1q and collagen domain-containing protein|adipocyte-specific protein AdipoQ|adiponectin d

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Adipoq, check out the Adipoq Infographic

Adipoq infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Adipoq: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0596 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tawfiq,R.A.,Attia,Y.M.,Nassar,N.N.,Hammam,O.A.,Elmazar,M.M.,& Abdallah,D.M.(2021).Insights into the Impact of FXR Activation on Hepatic Autophagy in a Non-alcoholic Steatohepatitis Model.Bulletin of Pharmaceutical Sciences.Assiut,44(1),265-274.
Species: Mouse
EK0596 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Trindade PL,Soares EDR,Inada KOP,Martins FF,Rudnicki M,Perrone D,Monteiro M,Souza-Mello V,Daleprane JB.Consumption of phenolic-rich jabuticaba (Myrciaria jaboticaba) powder ameliorates obesity-related disorders in mice.Br J Nutr.2021 Mar 31:1-30.doi:10.1017/S0007114521001136.Epub ahead of print.PMID:33787474.
Species: Mouse
EK0596 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI
Species: Mouse
EK0596 usage in article: APP:ELISA, SAMPLE:ADIPOSE TISSUE, DILUTION:NA

Oleic acid influences the adipogenesis of 3T3-L1 cells via DNA Methylation and may predispose to obesity and obesity-related disorders

Role of adiponectin in adipose tissue wound healing

Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model

Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet

Puerarin Protects Pancreatic ?-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine

Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice

Chen, M., Wang, X., Hu, Z., Zhou, H., Xu, Y., Qiu, L.,…, & Ying, Z. (2017). Programming of mouse obesity by maternal exposure to concentrated ambient fine particles. Particle and Fibre Toxicology, 14(1), 20. doi: 10.1186/s12989-017-0201-9

Chen S, Li J, Zhang Z, Li W, Sun Y, Zhang Q, Feng X, Zhu W. Can J Physiol Pharmacol. 2012 Feb;90(2):237-42. Doi: 10.1139/Y11-123. Epub 2012 Feb 6. Effects Of Resveratrol On The Amelioration Of Insulin Resistance In Kkay Mice.

Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).

Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...

Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...

Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...

Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.

Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

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1 Reviews For Mouse Adiponectin ELISA Kit PicoKine™

0

"Adiponectin Analysis in Mouse Serum and Culture Media"

Excellent

Source: Biocompare.com

"Adiponectin Analysis in Mouse Serum and Culture Media"

--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student

Application ELISA
Sample Serum, tissue homogenate
Detection --

"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")

Verified customer

Submitted

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9 Customer Q&As for Mouse Adiponectin ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-11-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-11-04

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

W. Hernandez

Verified customer

Asked: 2020-06-25

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-25

Question

Can this ELISA kit detect HMW and Total Adiponectin?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

This ELISA kit can detect HMW and Total Adiponectin.

Boster Scientific Support

Answered: 2019-06-12

Question

Can you confirm if the EK0596 is compatible with ELISA microplate washers?

Verified customer

Asked: 2019-05-30

Answer

Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.

Boster Scientific Support

Answered: 2019-05-30

Question

Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?

Verified customer

Asked: 2019-05-28

Answer

For the Mouse Adiponectin ELISA Kit PicoKine™ (EK0596), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.

Boster Scientific Support

Answered: 2019-05-29

Question

Q: how can I thaw whole blood sample for Adiponectin ELISA after freezing?

G. Johnson

Verified customer

Asked: 2018-09-05

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Adiponectin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-09-05

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

J. Sunder

Verified customer

Asked: 2018-05-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-05-13

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Adiponectin using the EK0596 Mouse Adiponectin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-04-26

Question

Q: What is the optimal O.D. value for Adiponectin ELISA kit? I used your Adiponectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Adiponectin even though the O.D. values are not very high?

B. Perez

Verified customer

Asked: 2018-01-09

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Adiponectin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Adiponectin that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-01-09

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