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Product Info Summary
| SKU: | M00522-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody
SKU/Catalog Number
M00522-1
BM4976 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody catalog # M00522-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00522-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAOO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AFP
Reactive Species
M00522-1 is reactive to AFP in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00522-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of AFP expression in HepG2 cell lysate.
Specific Publications For Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody (M00522-1)
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5 Customer Q&As for Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody
Question
We have observed staining in human heart. Are there any suggestions? Is anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody supposed to stain heart positively?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
According to literature heart does express AFP. According to Uniprot.org, AFP is expressed in embryo, heart, lung, brain cajal-retzius cell, among other tissues. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-05
Question
you antibody using your anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody for post-translational protein phosphorylation studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
We appreciate your inquiry. This M00522-1 anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-06
Question
Our team were well pleased with the WB result of your anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is AFP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
From literature, lung does express AFP. Generally AFP expresses in secreted. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-09
Question
We ordered your anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody for WB on brain cajal-retzius cell last year. I am using human, and We want to use the antibody for IP next. I am looking for examining brain cajal-retzius cell as well as heart in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
P. Evans
Verified customer
Asked: 2016-12-09
Answer
I viewed the website and datasheets of our anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody and I see that M00522-1 has been tested on human in both WB and IP. Thus M00522-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-12-09
Question
We are currently using anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody M00522-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
M. Rodriguez
Verified customer
Asked: 2013-01-02
Answer
The anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal antibody (M00522-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-02
