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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin or EDTA)|
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Human FUT8 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FUT8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FUT8 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FUT8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1990)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: R31-K575
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1990 is reactive to FUT8 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin or EDTA)
EK1990 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fucosyltransferase 8/FUT8
Alpha- (1,6)-fucosyltransferase is an enzyme that in humans is encoded by the FUT8 gene. It is mapped to 14q23.3. This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.
|EK1990-CAP||Anti-Human FUT8 Pre-coated 96-well strip microplate||1|
|EK1990-ST||Human FUT8 Standard||2 vials, 10 ng/tube|
|EK1990-DA||Human FUT8 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FUT8 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Fucosyltransferase 8/FUT8, Dilution ratio of 1:1, concentration in serum is around 5ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FUT8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Fucosyltransferase 8/FUT8 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FUT8 (Source: Uniprot.Org, NCBI)
glycosyltransferase 23 family
alpha 1,6 Fucosyltransferase; alpha-(1,6)-fucosyltransferase; alpha1-6FucT; EC 220.127.116.11; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); Fucosyltransferase 8; FUT8; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465 FUT8 CDGF, CDGF1 fucosyltransferase 8 alpha-(1,6)-fucosyltransferase|GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase|GDP-fucose--glycoprotein fucosyltransferase|alpha1-6FucT|fucosyltransferase 8 (alpha (1,6) fucosyltransferase)|glycoprotein 6-alpha-L-fucosyltransferase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FUT8, check out the FUT8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FUT8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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