Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband®

Product Name

Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband®

View all ANGPTL3 Antibodies

SKU/Catalog Number

PA2075
BA4015-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

ANGPTL3 is a secreted angiopoietin-like regulator of plasma lipid handling that suppresses lipoprotein lipase (LPL) activity in oxidative tissues, influencing routing/clearance of triglyceride-rich lipoproteins (VLDL-TG) (metabolic axis). Assay context: Human/Mouse/Rat-reactive antibody tested in WB for protein detection/relative abundance; commonly contextualized with intracellular lipid-activation enzymes such as ACSL5 and steroid-metabolism enzymes such as AKR1C1/C2 in metabolic phenotyping panels (putative co-panel).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2075)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ANGPTL3, identical to the related rat and mouse sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2075 is reactive to ANGPTL3 in Human, Mouse, Rat

Observed Molecular Weight

50 kDa

Calculated molecular weight

53.6 kDa

Background of ANGPTL3

ANGPTL3 (Angiopoietin-Like 3), also known as ANGPT5, is a protein which in humans is encoded by the ANGPTL3 gene. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. By radiation hybrid mapping and the use of surrounding genes, Conklin et al. (1999) mapped the ANGPTL3 gene to chromosome 1p31. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2075 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: A549 Cell

Validation Images & Assay Conditions

See full target information ANGPTL3

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