Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™

Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cx3cl1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0358
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™

See all CX3CL1/Fractalkine products

SKU/Catalog Number

EK0358

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cx3cl1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0358)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-T334

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0358 is reactive to Cx3cl1 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0358 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0358-CAP Anti-Rat Cx3cl1 Pre-coated 96-well strip microplate 1
EK0358-ST Rat Cx3cl1 Standard 2 vials, 20 ng/tube
EK0358-DA Rat Cx3cl1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0030.0710.1480.2760.4970.9271.4401.895

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)44818159967464194710491
Standard deviation32.776.23687.7235.7287.61912.71
CV (%)7.3%4.2%6.9%7.7%4.5%8.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CX3CL1/Fractalkine in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 144845240145043721.264.8%
Sample 218151598156518111697116.346.8%
Sample 399679196976191699523348.573.6%
*number of samples for each test n=16.

Gene/Protein Information For Cx3cl1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O55145

Gene ID

89808

Gene Name

Cx3cl1

Full Name

Fractalkine

Weight

41.977kDa

Superfamily

intercrine delta family

Alternative Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1 Cx3cl1|Cx3c, Scyd1|C-X3-C motif chemokine ligand 1|fractalkine|C-X3-C motif chemokine 1|CX3C membrane-anchored chemokine|chemokine (C-X3-C motif) ligand 1|neurotactin|small inducible cytokine subfamily D, 1|small-inducible cytokine D1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cx3cl1, check out the Cx3cl1 Infographic

Cx3cl1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cx3cl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0358 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhao, B., He, E., Pan, Y., Xu, H., & Song, X. (2017). Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain. Molecular Pain, 13, 1-10. doi: 10.1177/1744806917730254

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9 Customer Q&As for Rat Fractalkine/CX3CL1 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-12-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-14

Question

Q: how to analyze ELISA data? I have obtained CX3CL1 level in serum.

Y. Martin

Verified customer

Asked: 2020-04-05

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-04-05

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-25

Question

Q: how can I thaw whole blood sample for CX3CL1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-10-26

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CX3CL1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-10-26

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

J. Singh

Verified customer

Asked: 2018-07-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-07-22

Question

Q: if the enzyme conjugated CX3CL1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CX3CL1 antigen?

Verified Customer

Verified customer

Asked: 2018-05-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-05

Question

Q: can you recommend the dilution ratio of serum samples for detection of CX3CL1 in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

S. Hall

Verified customer

Asked: 2018-03-11

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CX3CL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-03-11

Question

Q: can I use citrate plasma as samples in Rat CX3CL1 Picokine® ELISA Kit (Catalog # EK0358)?

K. Roberts

Verified customer

Asked: 2018-01-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CX3CL1 causing disruption of its protein structure. CX3CL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CX3CL1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-26

Question

Q: What is the optimal O.D. value for CX3CL1 ELISA kit? I performed your CX3CL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CX3CL1 even though the O.D. values are not very high?

H. Walker

Verified customer

Asked: 2017-04-14

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CX3CL1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CX3CL1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-04-14

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