Click image to see more details
Product Info Summary
| SKU: | PA1511 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Aquaporin 8/AQP8 Antibody Picoband®
SKU/Catalog Number
PA1511
BA3619-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 8/AQP8 Antibody catalog # PA1511. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. AQP8 (aquaporin-8) is described as a water channel protein (aquaporin family of integral membrane proteins), with AQP8 mRNA reported on-page as detected in pancreas and colon. Assay context: validated for WB with an observed band at ~27 kDa. As an epithelial membrane transport marker, AQP8 readouts are often contextualized with other epithelial transport/metabolic markers such as ABCG5 (often tissue- and study-dependent; putative pairing).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 8/AQP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1511)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Aquaporin 8.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1511 is reactive to AQP8 in Human, Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.4 kDa
Background of AQP8
AQP8, also known as Aquaporin-8, is a protein that in humans is encoded by the AQP8 gene. Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. The AQP8 gene contains 6 exons. It is mapped to chromosome 16p12. Northern blot analysis of human tissues detected a 1.35-kb AQP8 mRNA only in pancreas and colon.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1511 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A431 whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.
Click image to see more details
Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.
Specific Publications For Anti-Aquaporin 8/AQP8 Antibody Picoband® (PA1511)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Aquaporin 8/AQP8 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Aquaporin 8/AQP8 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Aquaporin 8/AQP8 Antibody Picoband®
Question
We are currently using anti-Aquaporin 8/AQP8 antibody PA1511 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
B. Jackson
Verified customer
Asked: 2018-12-14
Answer
The anti-Aquaporin 8/AQP8 antibody (PA1511) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14
