Anti-Aquaporin 1/AQP1 Antibody Picoband®

Western blot analysis of Aquaporin 1/AQP1 using anti-Aquaporin 1/AQP1 antibody (A00865).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1/AQP1 antigen affinity purified polyclonal antibody (Catalog # A00865) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1/AQP1 at approximately 23 kDa. The expected band size for Aquaporin 1/AQP1 is at 23 kDa.

Aliskiren treatment prevented the reduction in water channel AQP2 expression in the obstructed kidneys of BUO and B-R1D mice. Semi-quantitative immunoblots probed with anti-AQP2, -AQP1, -AQP3, and -β-actin antibodies in the whole kidneys of BUO mice (A) and in the whole kidneys of B-R1D mice (C) . Corresponding densitometric analyses of AQP2, AQP1, and AQP3 protein expression corrected for β-actin in BUO mice (B) and in B-R1D mice (D) . Sham, sham-operated mice; BUO, bilateral ureteral obstruction for 24 h; BUO + Ali, BUO mice treated with aliskiren; B-R1D, bilateral ureteral obstruction for 16 h followed by 1-day release; B-R1D + Ali, B-R1D mice treated with aliskiren. ∗ p < 0.05 compared with the sham group; # p < 0.05 compared with the BUO/B-R1D group.
Index in PubMed under a CC BY license. PMID: 31572210

Aliskiren treatment prevented polyuria and the reduction in water channel AQP2 and AQP1 expression in the obstructed kidneys of B-R3D mice. (A) Urine output was collected from sham, B-R3D, and aliskiren-treated mice. (B) Semi-quantitative immunoblots probed with anti-AQP2, -AQP1, -AQP3, and β-actin antibodies. (C) Corresponding densitometric analyses of the AQP2, AQP1, and AQP3 protein expression corrected for β-actin. (D) Immunohistochemistry of AQP2 in the kidneys of aliskiren-treated or non-treated B-R3D mice. Sham, sham-operated groups; B-R3D, bilateral ureteral obstruction for 16 h followed by 3 days release; B-R3D + Ali, B-R3D group with aliskiren treatment. ∗ p < 0.05 compared with sham group; # p < 0.05 compared with B-R3D groups.
Index in PubMed under a CC BY license. PMID: 31572210