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- Table of Contents
ATF4 antibody (M00371) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-ATF4 Rabbit Monoclonal Antibody
ATF4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
ATF4 antibody was positively detected by IHC in:
mouse kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Mouse
Recommend
Tested
Recommend
Recommend
Recommend
Recommend
Human
Recommend
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-ATF4 Rabbit Monoclonal Antibody
View all ATF4 Antibodies
View all ATF4 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HCI-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATF4
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
38.6 kDa
Cite This Product
Anti-ATF4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00371)
Protocols
Immunohistochemistry (IHC) protocol for ATF4 antibody
Publications
Application:
Species:
Sodium butyrate induces mitochondrial pathway apoptosis in liver cancer via ATF4/SLC7A11-mediated ferroptosis
Published: 2026-07-15
Journal: PLoS One
Application: WB
Impact Factor: 2.80
Species: Human
PMID: 42455831
PCSK9 promotes aging-related cardiac calcification by inducing osteogenic differentiation of cardiac fibroblasts
Published: 2026-06-19
Journal: MECHANISMS OF AGEING AND DEVELOPMENT
Application: WB
Impact Factor: 6.50
Species: Mouse
PMID: 42320599
Methionine promotes cell proliferation in bovine mammary epithelial cells via the ATF4-GSK3β signalling pathway
Published: 2026-05-22
Journal: JOURNAL OF DAIRY RESEARCH
Application: IF,WB,CoIP
Impact Factor: 1.60
Species: Cow
PMID: 42170820
Preliminary exploration of endoplasmic reticulum stress transmission in astrocytes and neurons, and its mediators
Published: 2025-04-11
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 3.50
Species: Rat
PMID: 40242951
BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress
Published: 2024-07-07
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38973202
Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes
Published: 2023-11-07
Journal: NEUROCHEMICAL RESEARCH
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 37935859
Detection of the role of intestinal flora and tryptophan metabolism involved in antidepressant-like actions of crocetin based on a multi-omics approach
Published: 2022-09-28
Journal: PSYCHOPHARMACOLOGY
Application: WB
Impact Factor: 4.42
Species: Mouse
PMID: 36169685
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We promise Anti-ATF4 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00371
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 22, 2026