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- Table of Contents
ERN1 antibody (P00683) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Western blot analysis of P-IRE1 using anti-P-IRE1 antibody (P00683).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-6:Different intestinal tissues from pigs infected with classical swine fever.
electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-IRE1 antigen affinity purified monoclonal antibody (P00683) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for P-IRE1 at approximately 100-110 kDa. The expected band size for P-IRE1 is at 110 kDa.
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-6:Different intestinal tissues from pigs infected with classical swine fever.
electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-IRE1 antigen affinity purified monoclonal antibody (P00683) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for P-IRE1 at approximately 100-110 kDa. The expected band size for P-IRE1 is at 110 kDa.
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Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody
ERN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
ERN1 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human Raji whole cell, human 293T whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse RAW264.7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody
View all ERN1 Antibodies
View all ERN1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EGH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IRE1 (S724)
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
109.7 kDa
Cite This Product
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00683)
Protocols
Western Blot protocol for ERN1 antibody
Publications
Application:
Species:
Ketogenic diet attenuates cognitive dysfunctions induced by hypoglycemia via inhibiting endoplasmic reticulum stress-dependent pathways
Published: 2023-12-21
Journal: Food & Function
Application: WB
Impact Factor: 6.10
Species: Mouse
PMID: 38197246
ER-Stress and Senescence Coordinately Promote Endothelial Barrier Dysfunction in Diabetes-Induced Atherosclerosis
Published: 2022-07-06
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Human
PMID: 35889743
Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes
Published: 2020-06-18
Journal: Parasites & Vectors
Application: WB
Impact Factor: 2.82
Species: Mouse
PMID: 32552877
Review
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1 Review For Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody
The antibody shows a clear target band at the correct position and has strong affinity for phosphorylated IRE1 protein from pig.
| SKU | M00371 |
|---|---|
| Application | Western Blot |
| Sample | Porcine intestinal tissue |
| Sample Processing Description | Intestinal tissues from different segments of pigs infected with swine fever. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | ATF4 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | ATF4 is a key regulator of the integrated stress response. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, the expression of ATF4 is rapidly induced and upregulated. The aim of this experiment is to determine the expression levels of ATF4 protein in different intestinal segments infected with classical swine fever. |
Lu Jin, Zhejiang A&F University
Verified customer
Submitted 2025-12-03
Product Promise
We promise Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00683
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026