Product Info Summary
SKU: | M00346-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Atg7 (Apg7) Monoclonal Antibody
SKU/Catalog Number
M00346-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Atg7 (Apg7) Monoclonal Antibody catalog # M00346-1. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Atg7 (Apg7) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00346-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACHA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Atg7 (Apg7) Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00346-1 is reactive to ATG7 in Human, Mouse, Rat
Applications
M00346-1 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
77.96kDa
Background of ATG7
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Atg7(Apg7) expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)
Gene Name
ATG7
Full Name
Ubiquitin-like modifier-activating enzyme ATG7
Weight
77.96kDa
Superfamily
ATG7 family
Alternative Names
APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7 ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG7, check out the ATG7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Atg7 (Apg7) Monoclonal Antibody (M00346-1)
Hello CJ!
M00346-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Atg7 (Apg7) Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Atg7 (Apg7) Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question