Product Info Summary
SKU: | A01469 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BNIP3 Antibody Picoband®
SKU/Catalog Number
A01469
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BNIP3 Antibody Picoband® catalog # A01469. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BNIP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01469)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BNIP3, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01469 is reactive to BNIP3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
27.832kDa
Background of BNIP3
The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X (L) heterodimerization and confers pro-apoptotic activity; whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01469 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, rat kidney tissue, rat heart tissue, rat testis tissue, mouse brain tissue, mouse kidney tissue, mouse heart tissue, mouse testis tissue, human Hela whole cell, human A375 whole cell, human A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BNIP3 using anti-BNIP3 antibody (A01469).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3 antigen affinity purified polyclonal antibody (Catalog # A01469) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BNIP3 at approximately 21KD. The expected band size for BNIP3 is at 21KD.
Click image to see more details
Figure 2. Western blot analysis of BNIP3 using anti-BNIP3 antibody (A01469).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3 antigen affinity purified polyclonal antibody (Catalog # A01469) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BNIP3 at approximately 21KD. The expected band size for BNIP3 is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For BNIP3 (Source: Uniprot.org, NCBI)
Gene Name
BNIP3
Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Weight
27.832kDa
Superfamily
NIP3 family
Alternative Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3; NIP3 BNIP3 NIP3 BCL2 interacting protein 3 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3|BCL2/adenovirus E1B 19kDa interacting protein 3|BCL2/adenovirus E1B interacting protein 3|nineteen kD interacting protein-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BNIP3, check out the BNIP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BNIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BNIP3 Antibody Picoband® (A01469)
Hello CJ!
A01469 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao YC,Guo W,Gao BH. Hypoxic training upregulates mitochondrial turnover and angiogenesis of skeletal muscle in mice. Life Sci. 2021 Mar 11:119340.doi:10.1016/j.lfs.2021.119340.Epub ahead of print.PMID:33716067.
Species: Mouse
A01469 usage in article: APP:WB, SAMPLE:GASTROCNEMIUS TISSUE, DILUTION:1:1000
Du Y, Li J, Xu T, Zhou DD, Zhang L, Wang X. Oncotarget. 2017 Jun 22;8(37):61510-61527. doi: 10.18632/oncotarget.18604. eCollection 2017 Sep 22. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling
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