Anti-Fas Antibody Picoband™

Boster Bio Anti-Fas Antibody Picoband™ catalog # PB9252. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 20 publication(s).

Product Info Summary

SKU: PB9252
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Fas Antibody Picoband™

See all FAS products

SKU/Catalog Number

PB9252

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fas Antibody Picoband™ catalog # PB9252. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fas Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9252)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9252 is reactive to FAS in Human

Applications

PB9252 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For FAS (Source: Uniprot.Org, NCBI)

Uniprot ID

P25445

Gene ID

355

Gene Name

FAS

Full Name

Tumor necrosis factor receptor superfamily member 6

Weight

37.732kDa

Alternative Names

ALPS1A; APO-1 cell surface antigen; Apo-1; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; APT1; CD95 antigen; CD95; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6 FAS ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS Fas cell surface death receptor tumor necrosis factor receptor superfamily member 6|APO-1 cell surface antigen|CD95 antigen|FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas AMA|TNF receptor superfamily member 6|apoptosis antigen 1|apoptosis signaling receptor FAS|apoptosis-mediating surface antigen FAS|mutant tumor necrosis receptor superfamily member 6|tumor necrosis factor receptor superfamily, member 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FAS, check out the FAS Infographic

FAS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FAS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9252 has been cited in 20 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ferguson Bennit HR,Gonda A,Kabagwira J,Oppegard L,Chi D,Licero Campbell J,De Leon M,Wall NR.Natural Killer Cell Phenotype and Functionality Affected by Exposure to Extracellular Survivin and Lymphoma-Derived Exosomes.Int J Mol Sci.2021 Jan 27;22(3):1255.d
Species: Human
PB9252 usage in article: APP:FLOW CYTOMETRY, SAMPLE:EXOSOMES, DILUTION:NA

Osteoprotegerin Induces Apoptosis of Osteoclasts and Osteoclast Precursor Cells via the Fas/Fas Ligand Pathway

HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1

Construction and identification of Fas-targeting siRNA-expressing plasmid

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues

Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues

Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, Chen L, Liu C, Chen D, Chen L. Osteoarthritis Cartilage. 2014 Aug;22(8):1197-205. Doi: 10.1016/J.Joca.2014.06.031. Epub 2014 Jul 4. Loss Of Vhl In Cartilage Accelerated The Progression Of Age-Associated A...

Li W, Xia D, Wang Y, Li Y, Xue Y, Wu X, Ye Z. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):794-8. Doi: 10.1007/S11596-011-0679-6. Epub 2011 Dec 16. Relationship Between Aberrant Methylation Of Fas Promoter And Biological Behavior Of Bladd...

Liu Wl, Liu Zw, Li Ts, Wang C, Zhao B. Chin Med J (Engl). 2013 Feb;126(3):494-9. Hydrogen Sulfide Donor Regulates Alveolar Epithelial Cell Apoptosis In Rats With Acute Lung Injury.

Ma H, Zhang X, Zhang X, Yang D, Meng L, Zhang Y, Zhou S. Arch Med Sci. 2013 Apr 20;9(2):354-60. Doi: 10.5114/Aoms.2012.31439. Epub 2012 Oct 30. The Effect Of Esculentoside A On Lupus Nephritis-Prone Bxsb Mice.

Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Pro...

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

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16 Customer Q&As for Anti-Fas Antibody Picoband™

Question

I was wanting to use to test anti-Fas antibody PB9252 on human right ovary for research purposes, then I may be interested in using anti-Fas antibody PB9252 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

The products we sell, including anti-Fas antibody PB9252, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-28

Question

Would PB9252 anti-Fas antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

You can see on the product datasheet, PB9252 anti-Fas antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-24

Question

Is this PB9252 anti-Fas antibody reactive to the isotypes of FAS?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The immunogen of PB9252 anti-Fas antibody is E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-28

Question

We bought anti-Fas antibody for WB on cervix carcinoma last year. I am using human, and We intend to use the antibody for IHC next. you antibody examining cervix carcinoma as well as urinary bladder in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

I looked at the website and datasheets of our anti-Fas antibody and I see that PB9252 has been validated on human in both WB and IHC. Thus PB9252 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-22

Question

I was wanting to use your anti-Fas antibody for WB for human right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right ovary identification?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

It shows on the product datasheet, PB9252 anti-Fas antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-23

Question

Please see the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PB9252. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-13

Question

Do you have a BSA free version of anti-Fas antibody PB9252 available?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fas antibody PB9252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-21

Question

Will anti-Fas antibody PB9252 work for WB with right ovary?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

According to the expression profile of right ovary, FAS is highly expressed in right ovary. So, it is likely that anti-Fas antibody PB9252 will work for WB with right ovary.

Boster Scientific Support

Answered: 2019-09-26

Question

I am looking for using your anti-Fas antibody for fasl/ cd95l signaling studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

I appreciate your inquiry. This PB9252 anti-Fas antibody is tested on hela whole cell lysate, jurkat whole cell lysate, a549 whole cell lysate, smmc whole cell lysate, k562 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-28

Question

My question regarding product PB9252, anti-Fas antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9252 anti-Fas antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-24

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PB9252. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-30

Question

We are currently using anti-Fas antibody PB9252 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

The anti-Fas antibody (PB9252) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-05

Question

Is a blocking peptide available for product anti-Fas antibody (PB9252)?

T. Yang

Verified customer

Asked: 2017-06-07

Answer

We do provide the blocking peptide for product anti-Fas antibody (PB9252). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-07

Question

My team were satisfied with the WB result of your anti-Fas antibody. However we have seen positive staining in peripheral blood lymphocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is FAS supposed to be expressed?

K. Jackson

Verified customer

Asked: 2016-02-03

Answer

Based on literature, peripheral blood lymphocyte does express FAS. Generally FAS expresses in isoform 1: cell membrane, isoform 2: secreted., isoform 3: secreted., isoform 4: secreted., isoform 5: secreted., isoform 6: secreted. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-02-03

Question

I see that the anti-Fas antibody PB9252 works with WB, what is the protocol used to produce the result images on the product page?

V. Krishna

Verified customer

Asked: 2014-07-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-07-01

Question

We have observed staining in human cervix carcinoma. Do you have any suggestions? Is anti-Fas antibody supposed to stain cervix carcinoma positively?

J. Brown

Verified customer

Asked: 2014-06-19

Answer

According to literature cervix carcinoma does express FAS. According to Uniprot.org, FAS is expressed in right ovary, peripheral blood lymphocyte, urinary bladder, cervix carcinoma, liver, among other tissues. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-06-19

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PB9252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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