Product Info Summary
SKU: | PB9018 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ICAM1 Antibody Picoband™
View all ICAM-1/CD54 Antibodies
SKU/Catalog Number
PB9018
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ICAM1 Antibody Picoband™ catalog # PB9018. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9018)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse ICAM1 recombinant protein (Position: G198-P537). Mouse ICAM1 shares 74% amino acid (aa) sequence identity with rat ICAM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9018 is reactive to ICAM1 in Mouse, Rat
Applications
PB9018 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
57.825kDa
Background of ICAM-1/CD54
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ICAM1using anti-ICAM1 antibody (PB9018).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat NRK whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # PB9018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90-100KD. The expected band size for ICAM1 is at 58KD.
Click image to see more details
Figure 2. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)
Gene Name
ICAM1
Full Name
Intercellular adhesion molecule 1
Weight
57.825kDa
Superfamily
immunoglobulin superfamily
Alternative Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICAM1 Antibody Picoband™ (PB9018)
Hello CJ!
PB9018 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Silencing of central (Pro)renin receptor ameliorates salt-induced renal injury in CKD
Treatment of Low Molecular Weight Heparin Inhibits Systemic Inflammation and Prevents Endotoxin-Induced Acute Lung Injury in Rats
Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation
Zhang ZC,Zhou Q,Yang Y,Wang Y,Zhang JL.Highly Acylated Anthocyanins from Purple Sweet Potato ( Ipomoea batatas L.) Alleviate Hyperuricemia and Kidney Inflammation in Hyperuricemic Mice: Possible Attenuation Effects on Allopurinol. J Agric Food Chem.2019 Jun 5;67(22):6202-6211.doi:10.1021/acs.jafc.9b01810.Epub 2019 May 28.PMID:31091873.
Species: Mouse
PB9018 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Yi J,Tian M,Hu L,Kang N,Ma W,Zhi J,Zheng X,Ruan X,Gao M.The mechanisms of celastrol in treating papillary thyroid carcinoma based on network pharmacology and experiment verification.Ann Transl Med.2021 May;9(10):866.doi:10.21037/atm-21-1854.PMID:34164500;PMCID:PMC8184492.
Species: Human
PB9018 usage in article: APP:WB, SAMPLE:BCPAP CELL, DILUTION:1:1000
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
PB9018 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
PB9018 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA
Triptolide as an Alternative to IVIG Therapy for Kawasaki Disease in a Mouse Model
The hepatitis B virus X protein promotes hepatocellular carcinoma metastasis by upregulation of matrix metalloproteinases
A new selective vascular endothelial growth factor receptor 2 inhibitor ablates disease in a mouse model of psoriasis
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-ICAM1 Antibody Picoband™
Question
Is this PB9018 anti-ICAM1 antibody reactive to the isotypes of ICAM1?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of PB9018 anti-ICAM1 antibody is E.coli-derived mouse ICAM1 recombinant protein (Position: G198-P537). Mouse ICAM1 shares 74% amino acid (aa) sequence identity with rat ICAM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
We ordered your anti-ICAM1 antibody for WB on kidney in the past. I am using mouse, and I plan to use the antibody for IHC-P next. My lab would like examining kidney as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
R. Johnson
Verified customer
Asked: 2020-03-26
Answer
I looked at the website and datasheets of our anti-ICAM1 antibody and it seems that PB9018 has been tested on mouse in both WB and IHC-P. Thus PB9018 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-26
Question
We have seen staining in rat plasma. Are there any suggestions? Is anti-ICAM1 antibody supposed to stain plasma positively?
B. Jones
Verified customer
Asked: 2020-01-15
Answer
From what I have seen in literature plasma does express ICAM1. From what I have seen in Uniprot.org, ICAM1 is expressed in lung, kidney, blood, plasma, cervix carcinoma, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-ICAM1 antibody for IHC-P for rat erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
As indicated on the product datasheet, PB9018 anti-ICAM1 antibody has been validated for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-ICAM1 antibody (PB9018)?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We do provide the blocking peptide for product anti-ICAM1 antibody (PB9018). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-14
Question
I am interested in using your anti-ICAM1 antibody for cellular response to leukemia inhibitory factor studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you for your inquiry. This PB9018 anti-ICAM1 antibody is validated on mouse thymus tissue, tissue lysate, spleen tissue, rat thymus tissue, pc12 whole cell lysate, nrk whole cell lysate, rh35 whole cell lysate, liver tissue. It is guaranteed to work for IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-14
Question
Would PB9018 anti-ICAM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
It shows on the product datasheet, PB9018 anti-ICAM1 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-12
Question
Is there a BSA free version of anti-ICAM1 antibody PB9018 available?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ICAM1 antibody PB9018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-06
Question
We are currently using anti-ICAM1 antibody PB9018 for rat tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
The anti-ICAM1 antibody (PB9018) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-23
Question
I see that the anti-ICAM1 antibody PB9018 works with IHC-P, what is the protocol used to produce the result images on the product page?
A. Dhar
Verified customer
Asked: 2018-02-12
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-12
Question
I am interested in to test anti-ICAM1 antibody PB9018 on rat erythroleukemia for research purposes, then I may be interested in using anti-ICAM1 antibody PB9018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jha
Verified customer
Asked: 2018-01-17
Answer
The products we sell, including anti-ICAM1 antibody PB9018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-17
Question
My boss were well pleased with the WB result of your anti-ICAM1 antibody. However we have observed positive staining in plasma membrane using this antibody. Is that expected? Could you tell me where is ICAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
From what I have seen in literature, plasma does express ICAM1. Generally ICAM1 expresses in membrane. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2017-12-05
Question
Can you help my question with product PB9018, anti-ICAM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Parker
Verified customer
Asked: 2016-08-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9018 anti-ICAM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-04
Question
Will anti-ICAM1 antibody PB9018 work for IHC-P with erythroleukemia?
L. Krishna
Verified customer
Asked: 2014-07-29
Answer
According to the expression profile of erythroleukemia, ICAM1 is highly expressed in erythroleukemia. So, it is likely that anti-ICAM1 antibody PB9018 will work for IHC-P with erythroleukemia.
Boster Scientific Support
Answered: 2014-07-29
Question
Does anti-ICAM1 antibody PB9018 work on dog IHC-P with plasma?
E. Li
Verified customer
Asked: 2014-02-20
Answer
Our lab technicians have not tested anti-ICAM1 antibody PB9018 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ICAM1 antibody PB9018 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-02-20
Question
I have attached the WB image, lot number and protocol we used for erythroleukemia using anti-ICAM1 antibody PB9018. Please let me know if you require anything else.
C. Huang
Verified customer
Asked: 2014-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-ICAM1 antibody PB9018. Let me know if you need anything else.
S. Johnson
Verified customer
Asked: 2013-12-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-31