|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-ICAM-1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Intercellular adhesion molecule 1(ICAM1) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Cite This Product||Anti-ICAM-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9018)|
|Replacement Item||This antibody may replace the following items: sc-101702|sc-107|sc-135946|sc-1511|sc-1511-R|sc-18839|sc-18853|sc-18908|sc-31724|sc-31725|sc-366317|sc-390483|sc-53336|sc-71292|sc-7891|sc-8439 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived mouse ICAM1 recombinant protein (Position: G198-P537). Mouse ICAM1 shares 74% amino acid (aa) sequence identity with rat ICAM1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Intercellular adhesion molecule 1|
|Molecular Weight||58844 MW|
|Protein Function||ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). .|
|Tissue Specificity||Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.|
|Sequence Similarities||Belongs to the immunoglobulin superfamily. ICAM family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Intercellular adhesion molecule 1;ICAM-1;MALA-2;MyD10;CD54;Icam1;Icam-1;|
|Research Areas|||immunology|cell type markers|adhesion| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment|cell adhesion molecules| stem cells|hematopoietic progenitors|lymphoid|b lymphocytic lineage|t lymphocytic lineage| immunology|adaptive immunity|regulatory t cells||
Background for Intercellular adhesion molecule 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ICAM-1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ICAM-1 Antibody Images
Click the images to enlarge.
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Lung Tissue
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Rat Spleen Tissue
All lanes: Anti-ICAM1(PB9018) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 40ug
Lane 3: PC12 Whole Cell Lysate at 40ug
Lane 4: NRK Whole Cell Lysate at 40ug
Lane 5: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 90KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,