Anti-ICAM1 Antibody Picoband™

ICAM-1/CD54 antibody

Boster Bio Anti-ICAM1 Antibody Picoband™ catalog # PB9017. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PB9017
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-ICAM1 Antibody Picoband™

View all ICAM-1/CD54 Antibodies

SKU/Catalog Number

PB9017

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ICAM1 Antibody Picoband™ catalog # PB9017. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ICAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9017)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9017 is reactive to ICAM1 in Human

Applications

PB9017 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of ICAM-1/CD54

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)

Uniprot ID

P05362

Gene ID

3383

Gene Name

ICAM1

Full Name

Intercellular adhesion molecule 1

Weight

57.825kDa

Superfamily

immunoglobulin superfamily

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ICAM1, check out the ICAM1 Infographic

ICAM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9017 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of remifentanil on hemodynamics, liver function and ICAM-1 expression in liver cancer patients undergoing surgery

Activation of nuclear factor-kappa B and effects of pyrrolidine dithiocarbamate on TNBS-induced rat colitis

Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

Regulatory Peptides Modulate Adhesion of Polymorphonuclear Leukocytes to Bronchial Epithelial Cells through Regulation of Interleukins, ICAM-1 and NF-κB/IκB

Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects

Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion

Molecular immune mechanism of C‐phycocyanin from Spirulina platensis induces apoptosis in HeLa cells in vitro

Serum proteome analysis for profiling protein markers associated with carcinogenesis and lymph node metastasis in nasopharyngeal carcinoma

Preventative Effects of 4,4‐Diphenylmethane‐bis(methyl) Carbamate Isolated from Cortex Mori on Human Umbilical Vein Endothelial Cell Dysfunction Induced by Advanced Glycation End Products

Peoniflorin prevents the adhesion between inflammatory endothelial cells and leukocytes through inhibiting the activation of MAPKs and NF‐κB

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16 Customer Q&As for Anti-ICAM1 Antibody Picoband™

Question

I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-ICAM1 antibody PB9017. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ICAM1 antibody PB9017. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-31

Question

Is a blocking peptide available for product anti-ICAM1 antibody (PB9017)?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

We do provide the blocking peptide for product anti-ICAM1 antibody (PB9017). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-26

Question

I see that the anti-ICAM1 antibody PB9017 works with IHC-F, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-20

Question

I was wanting to use your anti-ICAM1 antibody for IHC-F for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

You can see on the product datasheet, PB9017 anti-ICAM1 antibody has been validated for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-20

Question

My lab would like using your anti-ICAM1 antibody for cellular response to lipopolysaccharide studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

Thanks for your inquiry. This PB9017 anti-ICAM1 antibody is tested on intestinal cancer tissue, hepg2 whole cell lysate, k562 whole cell lysate, a549 whole cell lysate, ht1080 whole cell lysate, a431 cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-22

Question

Would anti-ICAM1 antibody PB9017 work for IHC-F with leukemic t-cell?

J. Thomas

Verified customer

Asked: 2019-12-09

Answer

According to the expression profile of leukemic t-cell, ICAM1 is highly expressed in leukemic t-cell. So, it is likely that anti-ICAM1 antibody PB9017 will work for IHC-F with leukemic t-cell.

Boster Scientific Support

Answered: 2019-12-09

Question

Will PB9017 anti-ICAM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

As indicated on the product datasheet, PB9017 anti-ICAM1 antibody as been tested on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-25

Question

Is this PB9017 anti-ICAM1 antibody reactive to the isotypes of ICAM1?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The immunogen of PB9017 anti-ICAM1 antibody is E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-19

Question

We need to test anti-ICAM1 antibody PB9017 on human leukemic t-cell for research purposes, then I may be interested in using anti-ICAM1 antibody PB9017 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The products we sell, including anti-ICAM1 antibody PB9017, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-12

Question

We are currently using anti-ICAM1 antibody PB9017 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-08

Answer

The anti-ICAM1 antibody (PB9017) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-08

Question

Can you help my question with product PB9017, anti-ICAM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9017 anti-ICAM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-16

Question

We have been able to see staining in human erythroleukemia. Do you have any suggestions? Is anti-ICAM1 antibody supposed to stain erythroleukemia positively?

K. Jackson

Verified customer

Asked: 2018-03-13

Answer

Based on literature erythroleukemia does express ICAM1. Based on Uniprot.org, ICAM1 is expressed in lung, kidney, blood, plasma, cervix carcinoma, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2018-03-13

Question

Is there a BSA free version of anti-ICAM1 antibody PB9017 available?

Verified Customer

Verified customer

Asked: 2017-10-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ICAM1 antibody PB9017 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-13

Question

My boss were content with the WB result of your anti-ICAM1 antibody. However we have observed positive staining in leukemic t-cell membrane using this antibody. Is that expected? Could you tell me where is ICAM1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

From what I have seen in literature, leukemic t-cell does express ICAM1. Generally ICAM1 expresses in membrane. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2017-07-03

Question

Our lab used your anti-ICAM1 antibody for IF on kidney a few years ago. I am using human, and I plan to use the antibody for IHC-P next. I am interested in examining kidney as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?

S. Parker

Verified customer

Asked: 2015-06-04

Answer

I have checked the website and datasheets of our anti-ICAM1 antibody and it seems that PB9017 has been tested on human in both IF and IHC-P. Thus PB9017 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-06-04

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Total: $315

SKU:PB9017

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PB9017
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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