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ICAM1 antibody (PB9017) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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Flow Cytometry analysis of A431 cells using anti-ICAM1 antibody (PB9017).
Overlay histogram showing A431 cells stained with PB9017 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody (PB9017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PB9017 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody (PB9017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for ICAM1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ICAM1 Western Blot GuideBundle Save
Anti-ICAM1 Antibody Picoband®
ICAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
ICAM1 antibody was positively detected by WB in:
HEPG2 Whole Cell, K562 Whole Cell, A549 Whole Cell, HT1080 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ICAM1 antibody was positively detected by IHC in:
human intestinal cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ICAM1 antibody was positively detected by IF in:
human tonsil tissue, human Lung cancer tissue
ICAM1 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human
Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-ICAM1 Antibody Picoband®
View all ICAM1 Antibodies
View all ICAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
57.8 kDa
Background of ICAM1
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.
Cite This Product
Anti-ICAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9017)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
89%
Moderate Homology
Rabbit
62%
Low homology
Dog
59%
Low homology
Sheep
53%
Low homology
Cow
53%
Low homology
Mouse
52%
Low homology
Rat
50%
Low homology
Zebrafish
7%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ICAM1 antibody
Immunohistochemistry (IHC) protocol for ICAM1 antibody
Immunofluorescence (IF) protocol for ICAM1 antibody
Flow Cytometry (FC) protocol for ICAM1 antibody
Publications
Application:
Species:
Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB
Published: 2019-06-20
Journal: JOURNAL OF PHARMACOLOGICAL SCIENCES
Application: WB
Impact Factor: 2.44
Species: Human
PMID: 18946195
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
Published: 2017-07-12
Journal: Lipids in Health and Disease
Application: ICC
Impact Factor: 2.07
Species: Human
PMID: 28701173
Effects of remifentanil on hemodynamics, liver function and ICAM-1 expression in liver cancer patients undergoing surgery
Published: 2017-05-24
Journal: Oncology Letters
Application: IHC
Impact Factor: 1.39
Species: Human
PMID: 28693245
Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects
Published: 2017-04-13
Journal: JOURNAL OF FOOD SCIENCE
Application: WB
Impact Factor: 1.82
Species: Human
PMID: 28407238
Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion
Published: 2017-03-02
Journal: BIOSCIENCE REPORTS
Application: IHC
Impact Factor: 2.91
Species: Human
PMID: 28153917
Amelioration of compound 4,4′-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via RAGE/ERK1/2/NF-κB pathway
Published: 2012-12-05
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB,ICC
Impact Factor: 2.38
Species: Human
PMID: 23219582
Preventative Effects of 4,4'-Diphenylmethane-bis(methyl) Carbamate Isolated from Cortex Mori on Human Umbilical Vein Endothelial Cell Dysfunction Induced by Advanced Glycation End Products
Published: 2011-07-28
Journal: PHYTOTHERAPY RESEARCH
Application: ICC
Impact Factor: 1.88
Species: Human
PMID: 21796706
Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage
Published: 2011-06-06
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: ICC
Impact Factor: 3.12
Species: Human
PMID: 21647942
Molecular immune mechanism of C-phycocyanin from Spirulina platensis induces apoptosis in HeLa cells in vitro
Published: 2010-12-23
Journal: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Application: IHC
Impact Factor: 1.24
Species: Human
PMID: 16316316
Peoniflorin prevents the adhesion between inflammatory endothelial cells and leukocytes through inhibiting the activation of MAPKs and NF-κB
Published: 2010-06-11
Journal: DRUG DEVELOPMENT RESEARCH
Application: WB
Impact Factor: 0.67
Species: Human
Serum proteome analysis for profiling protein markers associated with carcinogenesis and lymph node metastasis in nasopharyngeal carcinoma
Published: 2008-03-21
Journal: CLINICAL & EXPERIMENTAL METASTASIS
Application: IHC
Impact Factor: 2.51
Species: Human
PMID: 18357507
Regulatory Peptides Modulate Adhesion of Polymorphonuclear Leukocytes to Bronchial Epithelial Cells through Regulation of Interleukins, ICAM-1 and NF-κB/IκB
Published: 2006-02-01
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: IHC
Impact Factor: 0.51
Species: Human
PMID: 16474903
Activation of nuclear factor-kappa B and effects of pyrrolidine dithiocarbamate on TNBS-induced rat colitis
Published: 2005-03-14
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Impact Factor: 0.00
PMID: 15770728
Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats.
Published: 2004-12-01
Journal: ACTA PHARMACOLOGICA SINICA
Impact Factor: 0.88
PMID: 15569408
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Product Promise
We promise Anti-ICAM1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9017
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026