Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

beta 2-Microglobulin antibody

Boster Bio Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10) catalog # M00456-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey.

Product Info Summary

SKU: M00456-2
Size: 100μg/vial
Reactive Species: Human, Monkey
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

View all beta 2-Microglobulin Antibodies

SKU/Catalog Number

M00456-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10) catalog # M00456-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00456-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

2H10

Isotype

IgG2b

Immunogen

E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00456-2 is reactive to B2M in Human, Monkey

Applications

M00456-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of beta 2-Microglobulin

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For B2M (Source: Uniprot.Org, NCBI)

Uniprot ID

P61769

Gene ID

567

Gene Name

B2M

Full Name

Beta-2-microglobulin

Weight

13.715kDa

Superfamily

beta-2-microglobulin family

Alternative Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin B2M IMD43 beta-2-microglobulin beta-2-microglobulin|beta chain of MHC class I molecules|beta-2-microglobin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on B2M, check out the B2M Infographic

B2M infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for B2M: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

Question

Do you have a BSA free version of anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 available?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-19

Question

We have seen staining in monkey dendritic cell. Do you have any suggestions? Is anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) supposed to stain dendritic cell positively?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

From literature dendritic cell does express B2M. From Uniprot.org, B2M is expressed in leukocyte, peripheral blood, cervix carcinoma, dendritic cell, leukemia, trachea, retina skin, plasma, urine, tear, liver, macrophage, among other tissues. Regarding which tissues have B2M expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Macrophage, Pubmed ID: 25356553
Peripheral blood, Pubmed ID: 16108498
Plasma, Pubmed ID: 8084451
Retina, and Skin, Pubmed ID: 15489334
Tear, Pubmed ID: 25946035
Trachea, Pubmed ID: 14702039
Urine, Pubmed ID: 7554280

Boster Scientific Support

Answered: 2020-02-12

Question

Our lab used your anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) for IHC on leukocyte last year. I am using human, and We intend to use the antibody for Flow Cytometry next. We need examining leukocyte as well as plasma in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

I looked at the website and datasheets of our anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) and it appears that M00456-2 has been validated on human in both IHC and Flow Cytometry. Thus M00456-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-03

Question

I have a question about product M00456-2, anti-Beta 2 Microglobulin antibody (monoclonal, 2H10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-16

Question

Would M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

As indicated on the product datasheet, M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-07

Question

Is a blocking peptide available for product anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) (M00456-2)?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

We do provide the blocking peptide for product anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) (M00456-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-24

Question

We are interested in to test anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 on human leukemia for research purposes, then I may be interested in using anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

The products we sell, including anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-17

Question

Is this M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) reactive to the isotypes of B2M?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

The immunogen of M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) is E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-25

Question

My boss were content with the WB result of your anti-Beta 2 Microglobulin antibody (monoclonal, 2H10). However we have seen positive staining in retina skin secreted using this antibody. Is that expected? Could you tell me where is B2M supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

From what I have seen in literature, retina skin does express B2M. Generally B2M expresses in secreted. Regarding which tissues have B2M expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Macrophage, Pubmed ID: 25356553
Peripheral blood, Pubmed ID: 16108498
Plasma, Pubmed ID: 8084451
Retina, and Skin, Pubmed ID: 15489334
Tear, Pubmed ID: 25946035
Trachea, Pubmed ID: 14702039
Urine, Pubmed ID: 7554280

Boster Scientific Support

Answered: 2019-06-20

Question

I have attached the WB image, lot number and protocol we used for leukemia using anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-04

Question

Will anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 work for Flow Cytometry with leukemia?

Verified Customer

Verified customer

Asked: 2018-09-17

Answer

According to the expression profile of leukemia, B2M is highly expressed in leukemia. So, it is likely that anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 will work for Flow Cytometry with leukemia.

Boster Scientific Support

Answered: 2018-09-17

Question

I was wanting to use your anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) for Flow Cytometry for human leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemia identification?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

As indicated on the product datasheet, M00456-2 anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) has been tested for Flow Cytometry, IHC, WB on human, monkey tissues. We have an innovator award program that if you test this antibody and show it works in human leukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-29

Question

We are currently using anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 for monkey tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, monkey. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

The anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) (M00456-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemia using anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2. Let me know if you need anything else.

C. Collins

Verified customer

Asked: 2017-04-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-04-20

Question

I see that the anti-Beta 2 Microglobulin antibody (monoclonal, 2H10) M00456-2 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

S. Singh

Verified customer

Asked: 2016-08-16

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-08-16

Conjugation

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Size

Total: $315

Size:100μg

SKU:M00456-2

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M00456-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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