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B2M antibody (M00456-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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8. Flow Cytometry analysis of A431 cells using anti-Beta 2 Microglobulin antibody (M00456-2).
Overlay histogram showing A431 cells stained with M00456-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beta 2 Microglobulin Antibody (M00456-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A431 cells stained with M00456-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beta 2 Microglobulin Antibody (M00456-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10)
B2M antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
B2M antibody was positively detected by WB in:
COS-7 whole cell, HELA whole cell, HL-60 whole cell, HEPG2 whole cell, K562 whole cell, 293T whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
B2M antibody was positively detected by IHC in:
human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
B2M antibody was positively detected by ICC/IF in:
A431 cell
B2M antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10)
View all B2M Antibodies
View all B2M Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2H10
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
12 kDa
Calculated Molecular Weight
13.7 kDa
Background of B2M
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Cite This Product
Anti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00456-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
91%
High Homology
Cow
76%
Moderate Homology
Sheep
76%
Moderate Homology
Rat
74%
Moderate Homology
Rabbit
74%
Moderate Homology
Dog
73%
Moderate Homology
Mouse
69%
Low homology
Zebrafish
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for B2M antibody
Immunohistochemistry (IHC) protocol for B2M antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for B2M antibody
Flow Cytometry (FC) protocol for B2M antibody
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Product Promise
We promise Anti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00456-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026