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ACTB antibody (M01263-4) performs well in IF, IHC, ICC, WB and shows reactivity with Chicken, Human, Mouse, Pig, Rabbit, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 63 Publications

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m01263 4 actb primary antibodies if testing 3

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Immunofluorescent analysis of HepG2 cells, using beta Actin mAb.

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Anti-beta Actin Mouse Monoclonal Antibody

ACTB antibody

63 Publications

Reactive Species	Host	Application
Chicken, Human, Mouse, Pig, Rabbit, Rat	Mouse	IF, IHC, ICC, WB

Tested Applications

ACTB antibody was positively detected by WB in:

human Hela whole cell, human K562 whole cell, pig brain tissue, rat brain tissue, mouse brain tissue, mouse NIH/3T3 whole cell, chicken liver tissuechicken brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTB antibody was positively detected by IHC in:

human heart tissue, rat liver tissue, rat NRK whole cell

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACTB antibody was positively detected by ICC/IF in:

HepG2 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Pig

Tested

Rat

Tested

Recommend

Mouse

Tested

Chicken

Tested

Product Information

Product Name

Anti-beta Actin Mouse Monoclonal Antibody
View all ACTB Antibodies

Form

Liquid

Uniprot

P60709

Gene ID

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Mouse IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

17A10

Isotype

IgG1

Immunogen

A synthesized peptide derived from human beta Actin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

41.7 kDa

Cite This Product

Anti-beta Actin Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-4)

Protocols

Western Blot protocol for ACTB antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACTB antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ACTB antibody

Review Protocol

Publications

Microplastic exposure is associated with gut microbiota alterations, intestinal barrier impairment, and endotoxin-related inflammation in female rats

Published: 2026-07-25

Journal: Current Research in Toxicology

Application: WB

Impact Factor: 4.70

Species: Rat

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Free Secondary Antibody

Anti-beta Actin Mouse Monoclonal Antibody

ACTB antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Microplastic exposure is associated with gut microbiota alterations, intestinal barrier impairment, and endotoxin-related inflammation in female rats

Anti-Aging Protein Klotho Attenuates Schizophrenia-Associated Cognitive and Synaptic Dysfunctions by Regulating GluN2B-NMDARs in the Hippocampus

Intracellular trafficking of furin enhances cellular intoxication by recombinant immunotoxins based on Pseudomonas exotoxin A

AKT1 and CTNNB1 mutations as drivers of paclitaxel resistance in breast cancer cells

CAR-γδ T Cells Targeting Claudin18.2 Show Superior Cytotoxicity Against Solid Tumor Compared to Traditional CAR-αβ T Cells

Folic acid intervention ameliorates hepatic steatosis after long-term alcohol exposure by alleviating endoplasmic reticulum stress

Dedifferentiated fat cells-derived exosomes (DFATs-Exos) loaded in GelMA accelerated diabetic wound healing through Wnt/β-catenin pathway

Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4

Vanillic acid ameliorates collagen-induced arthritis by suppressing the inflammation response via inhibition of the MAPK and NF-κB signaling pathways

Manshenkang granules alleviate fibrosis in chronic renal failure rats by regulating the PDE4b/cAMP pathway

Effect of Tristetraprolin on Esophageal Squamous Cell Carcinoma Cell Proliferation

O-GlcNAcylation attenuates ischemia-reperfusion-induced pulmonary epithelial cell ferroptosis via the Nrf2/G6PDH pathway

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Antitumor Effects of Tryptanthrin on Colorectal Cancer by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway and Targeting Topo I and IDO1

Effects of Bifidobacterium and rosuvastatin on metabolic-associated fatty liver disease via the gut–liver axis

Evaluation of immune and pyroptosis status in a model of sepsis-induced secondary pneumonia

Protective effect of andrographolide against ulcerative colitis by activating Nrf2/HO-1 mediated antioxidant response

miR-3202 inhibits bronchopulmonary dysplasia-mediated apoptosis and oxidative stress in bronchial epithelial cells via targeting RAG1

BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress

Polystyrene microplastics trigger testosterone decline via GPX1

Exploring the Anti-Inflammatory Effect of Tryptanthrin by Regulating TLR4/MyD88/ROS/NF-κB, JAK/STAT3, and Keap1/Nrf2 Signaling Pathways

MicroRNA-138 promotes the progression of multiple myeloma through targeting paired PAX5

Toll-like receptor 4 deficiency affects the balance of osteoclastogenesis and osteoblastogenesis in periodontitis

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Curcumin protects against cadmium-induced germ cell death in the testis of rats

p200CUX1-regulated BMP8B inhibits the progression of acute myeloid leukemia via the MAPK signaling pathway

CSE1L Silencing Enhances Cytarabine-mediated Cytotoxicity in Acute Myeloid Leukemia

Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling

S1PR2 participates in intestinal injury in severe acute pancreatitis by regulating macrophage pyroptosis

Fucoidan Improves D-Galactose-Induced Cognitive Dysfunction by Promoting Mitochondrial Biogenesis and Maintaining Gut Microbiome Homeostasis

THOC6 is a novel biomarker of glioma and a target of anti-glioma drugs: An analysis based on bioinformatics and molecular docking

Naodesheng Pills Ameliorate Cerebral Ischemia Reperfusion-Induced Ferroptosis via Inhibition of the ERK1/2 Signaling Pathway

Phoenixin 20 ameliorates pulmonary arterial hypertension via inhibiting inflammation and oxidative stress

Plumbagin Regulates Snail to Inhibit Hepatocellular Carcinoma Epithelial-Mesenchymal Transition in vivo and in vitro

Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury

Yellow leaf green tea modulates AMPK/ACC/SREBP1c signaling pathway and gut microbiota in high-fat diet-induced mice to alleviate obesity

Betulinic acid induces apoptosis of HeLa cells via ROS-dependent ER stress and autophagy in vitro and in vivo

Chondroitin sulfate/silk fibroin hydrogel incorporating graphene oxide quantum dots with photothermal-effect promotes type H vessel-related wound healing

Defective autophagic flux aggravates cadmium-induced Sertoli cell apoptosis

Chronic but not acute nicotine treatment ameliorates acute inflammation-induced working memory impairment by increasing CRTC1 and HCN2 in adult male mice

Evodiamine attenuates oxidative stress and ferroptosis by inhibiting the MAPK signaling to improve bortezomib-induced peripheral neurotoxicity

NF-κB Activator 1 downregulation in macrophages activates STAT3 to promote adenoma-adenocarcinoma transition and immunosuppression in colorectal cancer

125I seed implantation enhances arsenic trioxide-induced apoptosis and anti-angiogenesis in lung cancer xenograft mice

VEGF Modulates Neurogenesis and Microvascular Remodeling in Epileptogenesis After Status Epilepticus in Immature Rats

Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.

The protective effect of rutin against lipopolysaccharide induced acute lung injury in mice based on the pharmacokinetic and pharmacodynamic combination model

Adipose tissue alleviates the stress response by releasing adiponectin during laparoscopic surgery in patients with colorectal cancer

Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits

Effects of RAC1 on Proliferation of Hen Ovarian Prehierarchical Follicle Granulosa Cells

Cyanidin-3-glucoside protects liver from oxidative damage through AMPK/Nrf2 mediated signaling pathway in vivo and in vitro

Wnt4 negatively regulates the TGF-β1-induced human dermal fibroblast-to-myofibroblast transition via targeting Smad3 and ERK

Effects of early postnatal exposure to fine particulate matter on emotional and cognitive development and structural synaptic plasticity in immature and mature rats

HADHA overexpression disrupts lipid metabolism and inhibits tumor growth in clear cell renal cell carcinoma

Mitochondrial E3 ubiquitin ligase 1 promotes brain injury by disturbing mitochondrial dynamics in a rat model of ischemic stroke

1-calcium phosphate-uracil inhibits intraperitoneal metastasis by suppressing FAK in epithelial ovarian cancer

Effects of Wnt10b on dermal papilla cells via the canonical Wnt/β-catenin signalling pathway in the Angora rabbit

Downregulation of P2Y2 and HuD during the development of the enteric nervous system in fetal rats with anorectal malformations

Implication of SLIT3-ROBO1/ROBO2 in granulosa cell proliferation, differentiation and follicle selection in the prehierarchical follicles of hen ovary

High Concentration of Calcium Promotes Mineralization in NRK-52E Cells Via Inhibiting the Expression of Matrix Gla Protein

Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary

pH-sensitive micelles for the intracellular co-delivery of curcumin and Pluronic L61 unimers for synergistic reversal effect of multidrug resistance

Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells

Effect of RGD-modified silk material on the adhesion and proliferation of bone marrow-derived mesenchymal stem cells

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