Anti-Bid Il1b Antibody Picoband™

Boster Bio Anti-Bid Il1b Antibody Picoband™ catalog # PB9027. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PB9027
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Bid Il1b Antibody Picoband™

See all IL-1 beta/IL-1F2 products

SKU/Catalog Number

PB9027

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bid Il1b Antibody Picoband™ catalog # PB9027. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bid Il1b Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9027)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9027 is reactive to Il1b in Human, Mouse, Rat

Applications

PB9027 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For Il1b (Source: Uniprot.Org, NCBI)

Uniprot ID

Q63264

Gene ID

24494

Gene Name

Il1b

Full Name

Interleukin-1 beta

Weight

30.644kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Il1b|IL-1F2|interleukin 1 beta|interleukin-1 beta|IL-1 beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1b, check out the Il1b Infographic

Il1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9027 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ghoneim ME,Abdallah DM,Shebl AM,El-Abhar HS. The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy. Toxicol Appl Pharmacol.2020 Oct 29
Species: Rat
PB9027 usage in article: APP:WB, SAMPLE:PROTEIN EXTRACTIONS OF LIVER TISSUES, DILUTION:1:300

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Wang Xb, Liu Qh, Mi N, Wang P, Tang W, Zhao Xh, Li Xj. Ultrasound Med Biol. 2010 Apr;36(4):667-76. Doi: 10.1016/J.Ultrasmedbio.2009.09.006. Epub 2010 Mar 7. Sonodynamically Induced Apoptosis By Protoporphyrin Ix On Hepatoma-22 Cells In Vitro.

Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

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17 Customer Q&As for Anti-Bid Il1b Antibody Picoband™

Question

We need to test anti-Bid antibody PB9027 on rat blood for research purposes, then I may be interested in using anti-Bid antibody PB9027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

The products we sell, including anti-Bid antibody PB9027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-20

Question

We ordered your anti-Bid antibody for ICC on liver in the past. I am using rat, and We intend to use the antibody for Flow Cytometry next. We want examining liver as well as brain skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

I took a look at the website and datasheets of our anti-Bid antibody and it seems that PB9027 has been validated on rat in both ICC and Flow Cytometry. Thus PB9027 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-09

Question

I have a question about product PB9027, anti-Bid antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9027 anti-Bid antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-25

Question

Is a blocking peptide available for product anti-Bid antibody (PB9027)?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

We do provide the blocking peptide for product anti-Bid antibody (PB9027). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-29

Question

I see that the anti-Bid antibody PB9027 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-27

Question

Do you have a BSA free version of anti-Bid antibody PB9027 available?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bid antibody PB9027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-08

Question

Please see the WB image, lot number and protocol we used for blood using anti-Bid antibody PB9027. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-02

Question

Our lab want to know about using your anti-Bid antibody for positive regulation of neuron apoptotic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We appreciate your inquiry. This PB9027 anti-Bid antibody is tested on hela whole cell lysate, colo320 whole cell lysate, jurkat whole cell lysate, skov whole cell lysate. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-07

Question

Is this PB9027 anti-Bid antibody reactive to the isotypes of BID?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

The immunogen of PB9027 anti-Bid antibody is E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-14

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Bid antibody PB9027. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-27

Question

We are currently using anti-Bid antibody PB9027 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

C. Moore

Verified customer

Asked: 2018-10-11

Answer

The anti-Bid antibody (PB9027) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-11

Question

Will PB9027 anti-Bid antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-27

Answer

You can see on the product datasheet, PB9027 anti-Bid antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-27

Question

Does anti-Bid antibody PB9027 work on bovine IHC with blood?

H. Evans

Verified customer

Asked: 2017-03-23

Answer

Our lab technicians have not validated anti-Bid antibody PB9027 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Bid antibody PB9027 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-03-23

Question

I was wanting to use your anti-Bid antibody for Flow Cytometry for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?

L. Mitchell

Verified customer

Asked: 2015-12-09

Answer

You can see on the product datasheet, PB9027 anti-Bid antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-12-09

Question

We have seen staining in rat blood. What should we do? Is anti-Bid antibody supposed to stain blood positively?

J. Mitchell

Verified customer

Asked: 2014-01-28

Answer

From literature blood does express BID. From Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2014-01-28

Question

My team were well pleased with the WB result of your anti-Bid antibody. However we have been able to see positive staining in blood isoform 1: cytoplasm using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?

A. Li

Verified customer

Asked: 2013-09-03

Answer

From literature, blood does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2013-09-03

Question

Would anti-Bid antibody PB9027 work for Flow Cytometry with blood?

R. Singh

Verified customer

Asked: 2013-05-14

Answer

According to the expression profile of blood, BID is highly expressed in blood. So, it is likely that anti-Bid antibody PB9027 will work for Flow Cytometry with blood.

Boster Scientific Support

Answered: 2013-05-14

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PB9027
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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