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Product Info Summary
| SKU: | PB9725 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMP1 Antibody Picoband®
SKU/Catalog Number
PB9725
PB0764 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Bio Anti-MMP1 Antibody Picoband® catalog # PB9725. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Target: MMP1 (interstitial collagenase); function/pathways: extracellular matrix remodeling—cleaves collagens types I, II, III; biological/ disease context noted: roles in tissue remodeling with relevance to arthritis and metastasis; assay context supported here: WB. For integrated tumor biology panels, pairs with apoptosis regulator Survivin/BIRC5; immune-context comparisons can include Iba1/AIF1.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9725)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MMP1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9725 is reactive to MMP1 in Human
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.0 kDa
Background of MMP1
Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, is an enzyme that in humans is encoded by the MMP1 gene. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. It is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9725 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell, 22RV1 Whole Cell, MCF-7 Whole Cell, human placenta tissue, human PC-3 whole cell, human Caco-2 whole cell, human HEK293 whole cell, human THP-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MMP1 using anti-MMP1 antibody (PB9725).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SMMC Whole Cell Lysate,
Lane 2: 22RV1 Whole Cell Lysate,
Lane 3: MCF-7 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # PB9725) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54KD. The expected band size for MMP1 is at 54KD.
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Nti-inflammatory activity of PE against LPS-Induced M1 polarization in RAW264.7 macrophages. (A) Assessment of RAW264.7 cell viability after PE treatment; (B) Determination of the half-inhibitory concentration of PE on RAW264.7 cells (>200 μM) (n = 6); (C) Changes in cell morphology of RAW264.7 cells stimulated with LPS and treated with PE at concentrations of 8, 16, and 32 μM; (D) Measurement of fluorescence intensity of CD86 and INOS in RAW264.7 cells via immunofluorescence staining; (E,F) Quantification of fluorescence intensity from images (D) ; (G–K) Analysis of gene expression of INOS, IL-6, TNF-α, TGF-β, and MMP3 in RAW264.7 cells after modeling and PE treatment by RT-PCR; (L) Evaluation of protein expression of TNF-α, IL-6, MMP1, MMP9, and MMP13 in RAW264.7 cells after modeling and PE treatment; (M) Densitometric analysis of the protein expression shown in image (L) . Data are the mean ± SD of 3 independent experiments. # P < 0.05, ## P < 0.01, vs. Ctrl group; * P < 0.05, ** P < 0.01, vs. LPS group; ns, not significant.
Index in PubMed under a CC BY license. PMID: 41230092
Click image to see more details
Western blot analysis of MMP1 using anti-MMP1 antibody (PB9725).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # PB9725) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54KD. The expected band size for MMP1 is at 54KD.
Specific Publications For Anti-MMP1 Antibody Picoband® (PB9725)
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7 Customer Q&As for Anti-MMP1 Antibody Picoband®
Question
We were content with the WB result of your anti-MMP1 antibody. However we have been able to see positive staining in synovial cell secreted using this antibody. Is that expected? Could you tell me where is MMP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
According to literature, synovial cell does express MMP1. Generally MMP1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2020-05-06
Question
Is this PB9725 anti-MMP1 antibody reactive to the isotypes of MMP1?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The immunogen of PB9725 anti-MMP1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human MMP1 (194-231aa DAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTD). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-04
Question
We need to test anti-MMP1 antibody PB9725 on human ovary for research purposes, then I may be interested in using anti-MMP1 antibody PB9725 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The products we sell, including anti-MMP1 antibody PB9725, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-21
Question
We have been able to see staining in human synovial cell. Any tips? Is anti-MMP1 antibody supposed to stain synovial cell positively?
R. Rodriguez
Verified customer
Asked: 2019-06-14
Answer
Based on literature synovial cell does express MMP1. Based on Uniprot.org, MMP1 is expressed in smooth muscle tissue, ovary, synovial cell, fibroblast, among other tissues. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2019-06-14
Question
We need using your anti-MMP1 antibody for proteolysis studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
We appreciate your inquiry. This PB9725 anti-MMP1 antibody is tested on smmc whole cell lysate, 22rv1 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-22
Question
We are currently using anti-MMP1 antibody PB9725 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
D. Jones
Verified customer
Asked: 2017-02-01
Answer
The anti-MMP1 antibody (PB9725) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-01
Question
I was wanting to use your anti-MMP1 antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?
K. Jackson
Verified customer
Asked: 2013-05-30
Answer
As indicated on the product datasheet, PB9725 anti-MMP1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-30
