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Product Info Summary
| SKU: | M00334-4 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00334-4
BM4340 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody catalog # M00334-4. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DGI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human active + pro Caspase 3
Reactive Species
M00334-4 is reactive to CASP3 in Human, Mouse, Rat
Observed Molecular Weight
17,35 kDa
Calculated molecular weight
31.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00334-4 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human Jurkat whole cell,
rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
IHC: Human melanoma tissue, Human colon tissue, Human lung large cell cancer tissue, human stomach tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution.
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Western blot analysis of CASP3 using anti-CASP3 antibody (M00334-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 antigen affinity purified monoclonal antibody (Catalog # M00334-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3 at approximately 17,35 kDa. The expected band size for CASP3 is at 17,35 kDa.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody .
Specific Publications For Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (M00334-4)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Question
We are currently using anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-active + pro Caspase 3 Rabbit Monoclonal antibody (M00334-4) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
My question regarding product M00334-4, anti-active + pro Caspase 3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Singh
Verified customer
Asked: 2020-02-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-26
Question
I have attached the WB image, lot number and protocol we used for tongue using anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
My team were satisfied with the WB result of your anti-active + pro Caspase 3 Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?
T. Jackson
Verified customer
Asked: 2019-11-08
Answer
According to literature, cervix carcinoma erythroleukemia does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-08
Question
Is a blocking peptide available for product anti-active + pro Caspase 3 Rabbit Monoclonal antibody (M00334-4)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We do provide the blocking peptide for product anti-active + pro Caspase 3 Rabbit Monoclonal antibody (M00334-4). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-active + pro Caspase 3 Rabbit Monoclonal antibody for WB for human tongue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tongue identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody has been tested for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
My lab would like using your anti-active + pro Caspase 3 Rabbit Monoclonal antibody for neuron apoptotic process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate your inquiry. This M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody is tested on jurkat cell lysate, colo cells. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-09
Question
Is there a BSA free version of anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 available?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-28
Question
My question regards to test anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 on human tongue for research purposes, then I may be interested in using anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
The products we sell, including anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-06
Question
Will M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
You can see on the product datasheet, M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tongue using anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-23
Question
Would anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 work for WB with tongue?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
According to the expression profile of tongue, CASP3 is highly expressed in tongue. So, it is likely that anti-active + pro Caspase 3 Rabbit Monoclonal antibody M00334-4 will work for WB with tongue.
Boster Scientific Support
Answered: 2017-09-20
Question
We have observed staining in human t-cell. Any tips? Is anti-active + pro Caspase 3 Rabbit Monoclonal antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
Based on literature t-cell does express CASP3. Based on Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-08-28
Question
Is this M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody reactive to the isotypes of CASP3?
O. Zhang
Verified customer
Asked: 2014-08-11
Answer
The immunogen of M00334-4 anti-active + pro Caspase 3 Rabbit Monoclonal antibody is A synthesized peptide derived from human active + pro Caspase 3 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-11
Question
We ordered your anti-active + pro Caspase 3 Rabbit Monoclonal antibody for IP on cervix carcinoma erythroleukemia a few months ago. I am using mouse, and We want to use the antibody for IHC next. I was wanting to use examining cervix carcinoma erythroleukemia as well as tongue in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
T. Taylor
Verified customer
Asked: 2013-05-08
Answer
I have checked the website and datasheets of our anti-active + pro Caspase 3 Rabbit Monoclonal antibody and I see that M00334-4 has been validated on mouse in both IP and IHC. Thus M00334-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-05-08
