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- Table of Contents
CASP3 antibody (M00334-4) performs well in IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution.
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Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
CASP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IHC, WB |
Tested Applications
CASP3 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human Jurkat whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CASP3 antibody was positively detected by IHC in:
Human melanoma tissue, Human colon tissue, Human lung large cell cancer tissue, human stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Rat
Recommend
Product Information
Product Name
Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies
View all CASP3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DGI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human active + pro Caspase 3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17,35 kDa
Calculated Molecular Weight
31.6 kDa
Cite This Product
Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-4)
Protocols
Western Blot protocol for CASP3 antibody
Immunohistochemistry (IHC) protocol for CASP3 antibody
Publications
Application:
Species:
The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells
Published: 2025-07-01
Journal: Discover Oncology
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 40591050
Neochamaejasmin A Induces Apoptosis in Human Osteosarcoma MG-63 Cells via Mitochondrial Dysfunction and Phosphatidylinositol 3 Kinase/Protein Kinase B/Glycogen Synthase Kinase-3-Beta Signaling Pathway
Published: 2022-06-02
Journal: INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
Application: WB
Impact Factor: 0.66
Species: Human
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway
Published: 2022-03-08
Journal: JOURNAL OF NATURAL PRODUCTS
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 35255689
Transmembrane 4 L Six Family Member 1 Suppresses Hormone Receptor-–Positive, HER2-Negative Breast Cancer Cell Proliferation
Published: 2022-01-27
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.99
Species: Human
PMID: 35153775
Protective effects of mild hypothermia against hepatic injury in rats with acute liver failure
Published: 2019-05-06
Journal: Annals of Hepatology
Application: WB
Impact Factor: 1.90
Species: Rat
PMID: 31422029
Downregulation of long non-coding RNA Opa interacting protein 5-antisense RNA 1 inhibits breast cancer progression by targeting sex-determining region Y-box 2 by microRNA-129-5p upregulation
Published: 2018-11-15
Journal: CANCER SCIENCE
Application: WB
Impact Factor: 4.37
Species: Human
PMID: 30443959
microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2
Published: 2018-05-23
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 3.12
Species: Human
PMID: 29802821
Dexmedetomidine Alleviates Hyperoxia-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation
Published: 2017-08-03
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 5.10
Species: Rat,Mouse
PMID: 28873369
IL-10 Reduces Levels of Apoptosis in Toxoplasma gondii-Infected Trophoblasts
Published: 2013-02-13
Journal: PLoS One
Application: WB
Impact Factor: 3.73
Species: Human
PMID: 23418570
Inhibitory Effects of β,β-Dimethylacrylshikonin on Hepatocellular Carcinoma In Vitro and In Vivo
Published: 2011-11-23
Journal: PHYTOTHERAPY RESEARCH
Application: IHC
Impact Factor: 1.88
Species: Mouse
PMID: 22109831
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Product Promise
We promise Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00334-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026