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Product Info Summary
| SKU: | PA1513 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Caspase-10/CASP10 Antibody Picoband®
SKU/Catalog Number
PA1513
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-10/CASP10 Antibody catalog # PA1513. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-10/CASP10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1513)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CASP10.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1513 is reactive to CASP10 in Human
Observed Molecular Weight
57 kDa
Calculated molecular weight
59.0 kDa
Background of CASP10
Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction.This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1513 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: COLO320 Cell, HELA Cell, SW620 Cell, RAJI Cell
Validation Images & Assay Conditions
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Anti-Caspase-10 antibody, PA1513, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: RAJI Cell Lysate
Specific Publications For Anti-Caspase-10/CASP10 Antibody Picoband® (PA1513)
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Customer Q&As
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2 Customer Q&As for Anti-Caspase-10/CASP10 Antibody Picoband®
Question
We are currently using anti-Caspase-10/CASP10 antibody PA1513 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-Caspase-10/CASP10 antibody (PA1513) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
Question
I got a high background from the bands on my western blot gel. Here are the parameters that I used: - Protein loading 70 µg - SDS gel 10% - Electrophoresis 60V 60 min & 120V 95 min - Blocking 5% milk RT 30 min - Primary antibody 1:2000 dilution & 4°C overnight incubation - Secondary antibody α-Rabbit –HRP RT incubation for 1 hour. Please advise how we can lower the background.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Try to repeat your western blot with the following changes: - Protein loading 50 µg - Blocking 5% non-fat dry milk & incubate at 4°C for 2 hours with slow shaking - Primary antibody 1:4000 dilution. Then the background of your gel should be reduced.
Boster Scientific Support
Answered: 2020-02-20
