Anti-Interleukin-7 IL7 Antibody
Rabbit IgG polyclonal antibody for Interleukin-7(IL7) detection. Tested with WB, IHC-P in Human;Mouse.
Anti-Interleukin-7 IL7 Antibody Info At A Glance
|Product Name||Anti-Interleukin-7 IL7 Antibody
See all IL-7 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 7/IL7 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human;Mouse. IL 7/IL7 information: Molecular Weight: 17727 MW; Subcellular Localization: Secreted.|
|Cite This Product||Anti-Interleukin-7 IL7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1467)|
|Similar Products From
|Anti-Interleukin-7 IL7 Antibody may replace the following items: sc 34815|sc 34817|sc 34819|sc 365306|sc 4599|sc 73320|sc 7921.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of mouse IL7(26-42aa ECHIKDKEGKAYESVLM).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Il7 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||16196|
|Species Of This Entry||Mouse|
|Alternative Names||IL-7|IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Cleavage; Methylation; Dephosphorylation|
|Gene Location||3 A1|3 2.02 cM, on Chromasome 3, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il7 (Source: Uniprot.org, NCBI)
|Protein Function||Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.|
|Related Diseases||Hiv Infections; Malignant Neoplasms; Neoplasms; Sclerosis; Multiple Sclerosis; Immunologic Deficiency Syndromes; Lymphopenia; Leukemia; Inflammation; Lymphoma; Autoimmune Reaction; Graft-vs-host Disease; Infective Disorder; Autoimmune Diseases; Neoplasm Metastasis; Carcinoma; Acute Lymphocytic Leukemia; Virus Diseases; Hematologic Neoplasms|
|Related Pathways||Cell Development; Pathogenesis; Secretion; Cell Growth; Cell Proliferation; Immune Response; T Cell Homeostasis; Cell Activation; Cell Differentiation; Regeneration; Cytokine Production; T Cell Activation; T Cell Proliferation; Lymphocyte Homeostasis; Localization; Cell Death; Bone Resorption; Aging; Cytokine Secretion; Viral Replication|
|Background||IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.|
|Scientific References||PMID: 3259677 by Namen A.E., et al. Stimulation of B-cell progenitors by cloned murine interleukin-7.|
PMID: 2329282 by Lupton S.D., et al. Characterization of the human and murine IL-7 genes.
Our Boster Quality Guarantee for Anti-Interleukin-7 IL7 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Mouse Spleen Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL7 antigen affinity purified polyclonal antibody (Catalog # PA1467) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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