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Product Info Summary
| SKU: | PA1467 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Interleukin-7 IL7 Antibody Picoband®
SKU/Catalog Number
PA1467
BA4823 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-7 IL7 Antibody catalog # PA1467. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-7 IL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1467)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse IL7.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1467 is reactive to Il7 in Human, Mouse
Observed Molecular Weight
20 kDa
Calculated molecular weight
17.7 kDa
Background of Il7
IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1467 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human PC-3 whole cell
IHC: Mouse Spleen Tissue
Validation Images & Assay Conditions
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Anti-IL7 antibody, PA1467, IHC(P)
IHC(P): Mouse Spleen Tissue
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Western blot analysis of IL7 using anti-IL7 antibody (PA1467).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PA1467) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD.
Specific Publications For Anti-Interleukin-7 IL7 Antibody Picoband® (PA1467)
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5 Customer Q&As for Anti-Interleukin-7 IL7 Antibody Picoband®
Question
My boss were satisfied with the WB result of your anti-IL7 antibody. However we have been able to see positive staining in pancreas secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
From what I have seen in literature, pancreas does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-13
Question
We bought anti-IL7 antibody for WB on sperm in the past. I am using human, and I plan to use the antibody for IHC-P next. My question regards examining sperm as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
I viewed the website and datasheets of our anti-IL7 antibody and I see that PA1467 has been validated on human in both WB and IHC-P. Thus PA1467 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-06
Question
We have seen staining in human sperm. What should we do? Is anti-IL7 antibody supposed to stain sperm positively?
K. Li
Verified customer
Asked: 2015-07-30
Answer
Based on literature sperm does express IL7. Based on Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-30
Question
I was wanting to use using your anti-IL7 antibody for positive regulation of t cell differentiation studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
S. Dhar
Verified customer
Asked: 2014-08-29
Answer
Thank you for your inquiry. This PA1467 anti-IL7 antibody is tested on mouse spleen tissue. It is guaranteed to work for IHC-P, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-08-29
Question
We are currently using anti-IL7 antibody PA1467 for mouse tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
D. Collins
Verified customer
Asked: 2013-06-21
Answer
The anti-IL7 antibody (PA1467) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-21
