This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ARF1 antibody (M01279-1) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of ARF1 using anti-ARF1 antibody (M01279-1).
ARF1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ARF1 Antibody (M01279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ARF1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ARF1 Antibody (M01279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-ARF1 Rabbit Monoclonal Antibody
ARF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
ARF1 antibody was positively detected by WB in:
human 293T whole cell, human THP-1 whole cell, human PC-3 whole cell, human K562 whole cell, rat small intestines tissue, rat PC-12 whole cell, mouse small intestines tissue, mouse RAW264.7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ARF1 antibody was positively detected by IHC in:
human colon tissue, human colon cancer tissue, human thyroid cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-ARF1 Rabbit Monoclonal Antibody
View all ARF1 Antibodies
View all ARF1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GFA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ARF1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
20.7 kDa
Cite This Product
Anti-ARF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01279-1)
Protocols
Western Blot protocol for ARF1 antibody
Immunohistochemistry (IHC) protocol for ARF1 antibody
Review
Have you used Anti-ARF1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-ARF1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01279-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 29, 2026