Product Info Summary
| SKU: | PA1441-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-6(P18)/CASP6 Antibody Picoband®
SKU/Catalog Number
PA1441-1
BA3328 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-6 (P18)/CASP6 Antibody catalog # PA1441-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-6(P18)/CASP6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1441-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat Caspase-6(P18), different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1441-1 is reactive to Casp6 in Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
31.6 kDa
Background of Casp6
Caspase 6 is an enzyme that in humans is encoded by the caspase 6 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Using radiation hybrid mapping, the Caspase 6 gene is localized to human chromosome 4q25-q26. Caspase 6 functions as a downstream enzyme in the caspase activation cascade. And Caspase 6 can cleave lamin A to its signature apoptotic fragment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1441-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Rat Kidney Tissue, Rat Testis Tissue, NRK Cell, Mouse Liver Tissue, Mouse Kidney Tissue, Mouse Testis Tissue
IHC: Rat Spleen Tissue, Rat Intestine Tissue, Mouse Lung Tissue
IHC-F: Rat Spleen Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-6(P18) antibody, PA1441-1, IHC(P)
IHC(P): Rat Spleen Tissue
Click image to see more details
Anti-Caspase-6(P18) antibody, PA1441-1, IHC(P)
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-Caspase-6(P18) antibody, PA1441-1, IHC(P)
IHC(P): Mouse Lung Tissue
Click image to see more details
Anti-Caspase-6(P18) antibody, PA1441-1, IHC(F)
IHC(F): Rat Spleen Tissue
Click image to see more details
Anti-Caspase-6(P18) antibody, PA1441-1, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: NRK Cell Lysate
Lane 5: Mouse Liver Tissue Lysate
Lane 6: Mouse Kidney Tissue Lysate
Lane 7: Mouse Testis Tissue Lysate
Specific Publications For Anti-Caspase-6(P18)/CASP6 Antibody Picoband® (PA1441-1)
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3 Customer Q&As for Anti-Caspase-6(P18)/CASP6 Antibody Picoband®
Question
We are currently using anti-Caspase-6(P18)/CASP6 antibody PA1441-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The anti-Caspase-6(P18)/CASP6 antibody (PA1441-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-24
Question
I would like to test anti-Caspase-6(P18)/CASP6 antibody PA1441-1 on rat t-cell for research purposes, then I may be interested in using anti-Caspase-6(P18)/CASP6 antibody PA1441-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The products we sell, including anti-Caspase-6(P18)/CASP6 antibody PA1441-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-20
Question
Does anti-Caspase-6(P18)/CASP6 antibody PA1441-1 work on feline IHC with t-cell?
T. Baker
Verified customer
Asked: 2017-01-25
Answer
Our lab technicians have not validated anti-Caspase-6(P18)/CASP6 antibody PA1441-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-6(P18)/CASP6 antibody PA1441-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-25


