Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™

Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0453
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™

See all CCL20/MIP-3 alpha products

SKU/Catalog Number

EK0453

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0453)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A27-M96

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0453 is reactive to CCL20 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0453 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0453-CAP Anti-Human CCL20 Pre-coated 96-well strip microplate 1
EK0453-ST Human CCL20 Standard 2 vials, 10 ng/tube
EK0453-DA Human CCL20 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0530.1050.1710.3060.4780.9921.4672.144

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19782561773269
Standard deviation1.344.9113.051.225.0315.06
CV (%)7.1%6.9%5.6%7.2%6.9%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL20/MIP-3 alpha in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 119181919180.432.3%
Sample 278797274752.863.8%
Sample 32562622632632612.911.1%
*number of samples for each test n=16.

Gene/Protein Information For CCL20 (Source: Uniprot.Org, NCBI)

Uniprot ID

P78556

Gene ID

6364

Gene Name

CCL20

Full Name

C-C motif chemokine 20

Weight

10.762kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 CCL20 CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38 C-C motif chemokine ligand 20 C-C motif chemokine 20|CC chemokine LARC|beta chemokine exodus-1|chemokine (C-C motif) ligand 20|liver and activation-regulated chemokine|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20|small-inducible cytokine A20

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL20, check out the CCL20 Infographic

CCL20 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL20: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0453 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation
Species: Human

The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway

Li D, Wang A, Liu X, Meisgen F, Gr??nler J, Botusan Ir, Narayanan S, Erikci E, Li X, Blomqvist L, Du L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina Sb, St??hle M, Land??n Nx. J Clin Invest. 2015 Aug 3;125(8):3008-26. Doi: 10.1172/Jci79052. Epub 20...

Calvayrac O, Rodr??guez-Calvo R, Mart??-Pamies I, Alonso J, Ferr??n B, Aguil?? S, Crespo J, Rodr??guez-Sinovas A, Rodr??guez C, Mart??nez-Gonz??lez J. J Mol Cell Cardiol. 2015 Mar;80:34-44. Doi: 10.1016/J.Yjmcc.2014.12.015. Epub 2014 Dec 20. Nor-1...

Chen D, Hu Q, Mao C, Jiao Z, Wang S, Yu L, Xu Y, Dai D, Yin L, Xu H. Cell Immunol. 2012;272(2):166-74. Doi: 10.1016/J.Cellimm.2011.10.015. Epub 2011 Oct 28. Increased Il-17-Producing Cd4(+) T Cells In Patients With Esophageal Cancer.

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8 Customer Q&As for Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for CCL20 ELISA kit? I performed your CCL20 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CCL20 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-08-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL20 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CCL20 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2020-08-07

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-06-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-05-15

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-05-15

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CCL20 using the EK0453 Human CCL20 Picokine® ELISA Kit?

N. Zhao

Verified customer

Asked: 2019-11-04

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-11-04

Question

Q: how to thaw whole blood sample for CCL20 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL20 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-07-16

Question

Q: how to analyze ELISA data? I have performed CCL20 level in plasma.

W. Phillips

Verified customer

Asked: 2018-06-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-14

Question

Q: if the enzyme conjugated CCL20 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL20 antigen?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-07-10

Question

Q: Can CCL20 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-01-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-01-01

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