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Product Info Summary
| SKU: | M00144 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD11b ITGAM Rabbit Monoclonal Antibody
SKU/Catalog Number
M00144
BM4092 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b ITGAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00144)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD11b
Reactive Species
M00144 is reactive to ITGAM in Human
Observed Molecular Weight
170 kDa
Calculated molecular weight
127.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00144 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: TF1 cell
IHC: human colon carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of CD11b expression in TF1 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody.
Specific Publications For Anti-CD11b ITGAM Rabbit Monoclonal Antibody (M00144)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Question
Our team were well pleased with the WB result of your anti-CD11b Rabbit Monoclonal antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
According to literature, blood does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-06-28
Question
Our lab used your anti-CD11b Rabbit Monoclonal antibody for WB on bone marrow cell in the past. I am using human, and We intend to use the antibody for ICC next. My question regards examining bone marrow cell as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
I took a look at the website and datasheets of our anti-CD11b Rabbit Monoclonal antibody and it seems that M00144 has been tested on human in both WB and ICC. Thus M00144 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-20
Question
We are currently using anti-CD11b Rabbit Monoclonal antibody M00144 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
L. Johnson
Verified customer
Asked: 2017-06-20
Answer
The anti-CD11b Rabbit Monoclonal antibody (M00144) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-20
Question
We have seen staining in human blood. Do you have any suggestions? Is anti-CD11b Rabbit Monoclonal antibody supposed to stain blood positively?
M. Zhao
Verified customer
Asked: 2014-01-31
Answer
From what I have seen in literature blood does express ITGAM. From what I have seen in Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2014-01-31
