Mouse CD22/Siglec 2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD22. 96wells/kit, with removable strips.
Mouse CD22/Siglec 2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse CD22/Siglec 2 ELISA Kit PicoKine™
See all Siglec-2/CD22 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Siglec-2/CD22 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Siglec-2/CD22 tissue specificity: B-lymphocytes.|
|Cite This Product||Mouse CD22/Siglec 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1303)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: S22-R702|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Cd22 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||12483|
|Species Of This Entry||Mouse|
|Protein Name||B-cell receptor CD22|
|Alternative Names||Siglec-2/CD22|B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Dephosphorylation; Deglycosylation; Biotinylation; Acetylation; Ribosylation; Oxidation; Iodination; Methylation; Sulphation; Reduction|
|Gene Location||7 B1|7 19.26 cM, on Chromasome 7, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cd22 (Source: Uniprot.org, NCBI)
|Protein Function||Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.|
|Research Areas||Adaptive Immunity; DNA Repair; Immunology|
|Related Diseases||Leukemia; Lymphoma; Neoplasms; B-cell Lymphomas; Malignant Neoplasms; Lymphoid Leukemia; Acute Lymphocytic Leukemia; Chronic Lymphocytic Leukemia; Lymphoma, Non-hodgkin; Tissue Adhesions; Malignant Paraganglionic Neoplasm; Burkitt Lymphoma; Hairy Cell Leukemia; Autoimmune Reaction; Autoimmune Diseases; Leukemia, Myelocytic, Acute; Lupus Erythematosus, Systemic; Myeloid Leukemia; Leukemia, B-cell; Autoimmunity|
|Related Pathways||Cell Activation; Pathogenesis; B Cell Activation; Cell Adhesion; Cell Development; Immune Response; Cell Differentiation; Cell Death; Localization; Cell Proliferation; Cell Cycle; B Cell Differentiation; Lymphocyte Activation; Secretion; Glycosylation; B Cell Proliferation; Cytokine Production; Cell Maturation; Endocytosis; Cell Growth|
|Background||CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors(BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.|
|Scientific References||PMID: 8100843 by Law C.-L., et al. Organization of the murine Cd22 locus. Mapping to chromosome 7 and characterization of two alleles.|
PMID: 10501843 by Lajaunias F., et al. Polymorphisms in the Cd22 gene of inbred mouse strains.
Kit Components And Assay QC Data Details Of Mouse CD22/Siglec 2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1303-CAP||96-well plate precoated with anti-Mouse Siglec-2/CD22 antibody||1|
|EK1303-ST||lyophilized recombinant Mouse Siglec-2/CD22 standard||10ng/tube|
|EK1303-DA||biotinylated anti-Mouse Siglec-2/CD22 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1303 Mouse CD22/Siglec 2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse CD22/Siglec 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CD22/Siglec 2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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