Mouse CD22/Siglec 2 ELISA Kit PicoKine™

Mouse CD22/Siglec 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1303
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD22/Siglec 2 ELISA Kit PicoKine™

See all Siglec-2/CD22 products

SKU/Catalog Number

EK1303

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD22/Siglec 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD22/Siglec 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1303)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-R702

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1303 is reactive to Cd22 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1303 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1303-CAP Anti-Mouse Cd22 Pre-coated 96-well strip microplate 1
EK1303-ST Mouse Cd22 Standard 2 vials, 10 ng/tube
EK1303-DA Mouse Cd22 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010,000
O.D.0.0390.0800.1160.2210.3940.7011.1501.992

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD22/Siglec 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2402070405824619143971
Standard deviation16.56124.2182.6118.94132.06218.4
CV (%)6.9%6%4.5%7.7%6.9%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Siglec-2/CD22 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12402422512622488.693.5%
Sample 220701778177921201936159.238.2%
Sample 340584096475444604342284.976.5%
*number of samples for each test n=16.

Gene/Protein Information For Cd22 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35329

Gene ID

12483

Gene Name

Cd22

Full Name

B-cell receptor CD22

Weight

96.582kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 Cd22|A530093D23, Lyb, Lyb-, Lyb-8, Lyb8|CD22 antigen|B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2|siglec-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd22, check out the Cd22 Infographic

Cd22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse CD22/Siglec 2 ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD22 using the EK1303 Mouse CD22 Picokine® ELISA Kit?

J. Evans

Verified customer

Asked: 2020-02-15

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-02-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-04-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-04-28

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-01-18

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-01-18

Question

Q: how to analyze ELISA data? I measured CD22 level in plasma.

Verified Customer

Verified customer

Asked: 2018-11-18

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-11-18

Question

Q: if the enzyme conjugated CD22 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD22 antigen?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-02

Question

Q: how to thaw whole blood sample for CD22 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD22 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-06-08

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