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Product Info Summary
| SKU: | A01513 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD31 PECAM1 Antibody
SKU/Catalog Number
A01513
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CD31 PECAM1 Antibody (Catalog # A01513). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CD31 PECAM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01513)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01513 is reactive to PECAM1 in Human, Mouse
Calculated molecular weight
76.2 kDa
Background of PECAM1
Anti-CD31 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD31 (PECAM-1) is a 130 kDa platelet endothelial cell adhesion molecule encoded by the PECAM1 gene found on chromosome 17 in humans. CD31 is expressed on platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 plays a key role in modulation of integrin-mediated cell adhesion, leukocyte transendothelial migration, angiogenesis, apoptosis and macrophage phagocytosis. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Anti-CD31 is ideal for researchers interested in Stem Cell Research, Epigenetics, and Cell-cycle Regulation research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01513 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000
IHC: 4µg/mL
WB: 1:500
CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Validation Images & Assay Conditions
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Western Blot of Rabbit Anti-CD31 Antibody. Lane 1: 3T3 Whole cell lysate . Lane 2: Jurkat Whole cell lysate . Lane 3: HT-29 Whole cell lysate . Load: 10µg/lane. Primary Antibody Anti-CD-31 used 1:500 with 0.75% TBS with Casein overnight. Secondary Antibody Goat anti-rabbit HRP used 1:40,000 for 30 min at room temp. Expect MW: 80kda.
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Immunohistochemistry with anti-CD31 antibody showing CD31 staining of vascular endothelium in human colon at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human CD31 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).
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SH inhibits angiogenesis and promotes vessel normalization. (A) Staining for CD31 (red) showing the vessel area (CD31 + area, %) and vessel diameters in tumors. (B) Staining for FITC-conjugated lectin (green) and CD31 (red) indicating perfused lectin + CD31 + vessels (% of CD31 + vessels) in tumors. (C) The tumor oxygen supply was determined by HIF-1α staining (HIF-1α + /total cells). (D) HE staining showing necrosis (necrotic area is marked with red lines) in tumors. (E) Double staining for CD31 (red) and α-SMA (green) showing pericyte-covered tumor vessels within tumors which is further emphasized by confocal microscopy analysis of double staining of thick tumor sections followed by 3D projection of z-slice images (F). (G) Tumor volumes in mice injected intravenously with a suboptimal dose of doxorubicin (Doxo) or saline combined with SH administration. Bars: 100 μm. Statistical significance: P < 0.05 (*), P < 0.01 (**) or P < 0.001 (***), N = 6–8.
Index in PubMed under a CC BY license. PMID: 25749075
Specific Publications For Anti-CD31 PECAM1 Antibody (A01513)
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16 Customer Q&As for Anti-CD31 PECAM1 Antibody
Question
I was wanting to use your anti-CD31 antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
You can see on the product datasheet, A01513 anti-CD31 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-23
Question
Please see the WB image, lot number and protocol we used for brain using anti-CD31 antibody A01513. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-CD31 antibody A01513 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-16
Question
Do you have a BSA free version of anti-CD31 antibody A01513 available?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD31 antibody A01513 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-30
Question
We have observed staining in human brain. Any tips? Is anti-CD31 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
From literature brain does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-02-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CD31 antibody A01513. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
Our lab want to know about to test anti-CD31 antibody A01513 on human brain for research purposes, then I may be interested in using anti-CD31 antibody A01513 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The products we sell, including anti-CD31 antibody A01513, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-CD31 antibody A01513 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-CD31 antibody (A01513) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
My question regarding product A01513, anti-CD31 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01513 anti-CD31 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
Would A01513 anti-CD31 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
It shows on the product datasheet, A01513 anti-CD31 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-08
Question
My colleagues were well pleased with the WB result of your anti-CD31 antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Based on literature, brain does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-06-18
Question
I am looking for using your anti-CD31 antibody for diapedesis studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysates? We would like to see some validation images before ordering.
K. Jha
Verified customer
Asked: 2019-06-03
Answer
We appreciate your inquiry. This A01513 anti-CD31 antibody is validated on nih3t3 whole cell lysates, jurkat whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-03
Question
Is a blocking peptide available for product anti-CD31 antibody (A01513)?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
We do provide the blocking peptide for product anti-CD31 antibody (A01513). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-03-12
Question
Is this A01513 anti-CD31 antibody reactive to the isotypes of PECAM1?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
The immunogen of A01513 anti-CD31 antibody is This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-29
Question
Would anti-CD31 antibody A01513 work for IHC with brain?
J. Miller
Verified customer
Asked: 2016-09-21
Answer
According to the expression profile of brain, PECAM1 is highly expressed in brain. So, it is likely that anti-CD31 antibody A01513 will work for IHC with brain.
Boster Scientific Support
Answered: 2016-09-21
Question
We ordered your anti-CD31 antibody for WB on tendon of biceps brachii in a previous project. I am using rat, and I plan to use the antibody for IHC next. I am interested in examining tendon of biceps brachii as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
N. Thomas
Verified customer
Asked: 2013-09-11
Answer
I have checked the website and datasheets of our anti-CD31 antibody and it appears that A01513 has been validated on rat in both WB and IHC. Thus A01513 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-09-11
