Rat Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Rat Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Lgals1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml . Cited in 1 publication(s).

Product Info Summary

SKU: EZ1589
Size: For 5 plates, 96 wells each
Reactive Species: Rat
Application: ELISA

Product Name

Rat Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Galectin-1 products

SKU/Catalog Number

EZ1589

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Rat Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Lgals1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1589)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-E135

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1589 is reactive to Lgals1 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1589 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1589-CA Rabbit anti- rat Lgals1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1589-DA Biotinylated goat anti- rat Lgals1 polyclonal antibody (Detection Antibody) 500 µl, 3.3 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1589-ST Lyophilized recombinant rat Lgals1 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631362512502500500010000
O.D.0.0790.1740.2580.4070.6841.0561.572.274

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2431504543726215125561
Standard deviation15.3185.71260.9817.29110.4428.2
CV (%)6.3%5.7%4.8%6.6%7.3%7.7%

Gene/Protein Information For Lgals1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P11762

Gene ID

56646

Gene Name

Lgals1

Full Name

Galectin-1

Weight

14.857kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 Lgals1|galectin 1|galectin-1|14 kDa lectin|RL 14.5|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|gal-1|galaptin|lactose-binding lectin 1|lectin, galactose binding, soluble 1|lectin, galactoside-binding, soluble, 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lgals1, check out the Lgals1 Infographic

Lgals1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lgals1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EZ1589 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma

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