Product Info Summary
SKU: | PB9415 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ABCG5 Antibody Picoband™
SKU/Catalog Number
PB9415
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCG5 Antibody Picoband™ catalog # PB9415. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCG5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9415)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9415 is reactive to Abcg5 in Human
Applications
PB9415 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
73 kDa
Calculated molecular weight
73.244kDa
Background of Abcg5
ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCG5 using anti-ABCG5 antibody (PB9415).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug,
Lane 2: A549 Whole Cell Lysate at 40ug,
Lane 3: PANC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PB9415) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73 kDa. The expected band size for ABCG5 is at 73 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Abcg5 (Source: Uniprot.org, NCBI)
Gene Name
Abcg5
Full Name
ATP-binding cassette sub-family G member 5
Weight
73.244kDa
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; ATP-binding cassette, subfamily G, member 5; sterolin 1; sterolin-1; STSL Abcg5|Ster, cm, cmp, sterolin-1, tr, trac|ATP binding cassette subfamily G member 5|ATP-binding cassette sub-family G member 5|ATP-binding cassette, sub-family G (WHITE), member 5|sterolin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Abcg5, check out the Abcg5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Abcg5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCG5 Antibody Picoband™ (PB9415)
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No publications found for PB9415
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Customer Q&As
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1 Customer Q&As for Anti-ABCG5 Antibody Picoband™
Question
We are currently using anti-ABCG5 antibody PB9415 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-ABCG5 antibody (PB9415) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21