Anti-ABCG5 Antibody Picoband™

Abcg5 antibody

Boster Bio Anti-ABCG5 Antibody Picoband™ catalog # PB9415. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9415
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ABCG5 Antibody Picoband™

View all Abcg5 Antibodies

SKU/Catalog Number

PB9415

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCG5 Antibody Picoband™ catalog # PB9415. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCG5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9415)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9415 is reactive to Abcg5 in Human

Applications

PB9415 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

73.244kDa

Background of Abcg5

ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Abcg5 (Source: Uniprot.org, NCBI)

Gene Name

Abcg5

Full Name

ATP-binding cassette sub-family G member 5

Weight

73.244kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; ATP-binding cassette, subfamily G, member 5; sterolin 1; sterolin-1; STSL Abcg5|Ster, cm, cmp, sterolin-1, tr, trac|ATP binding cassette subfamily G member 5|ATP-binding cassette sub-family G member 5|ATP-binding cassette, sub-family G (WHITE), member 5|sterolin 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Abcg5, check out the Abcg5 Infographic

Abcg5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Abcg5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-ABCG5 Antibody Picoband™

Question

We are currently using anti-ABCG5 antibody PB9415 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

The anti-ABCG5 antibody (PB9415) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-21

Size

Conjugation

Total: $370

SKU:PB9415

In stock, 3+ left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

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