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Product Info Summary
| SKU: | M04577 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC |
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Product info
Product Name
Anti-CD370 Purified CLEC9A Monoclonal Antibody
SKU/Catalog Number
M04577
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD370 Purified CLEC9A Monoclonal Antibody (Catalog# M04577). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-CD370 Purified CLEC9A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04577)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
8F9
Isotype
Mouse IgG2a
Immunogen
RBL-2H3 cells expressing human CLEC9A fused to an HA epitope. The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04577 is reactive to CLEC9A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
27.3 kDa
Background of CLEC9A
CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04577 is guaranteed for Flow Cytometry, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Flow cytometry multicolor surface staining of human myeloid cells stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml, GAM FITC) and anti-human CD16 (3G8) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD370 positive CD16 positive dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml) GAM FITC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml) GAM FITC.
Specific Publications For Anti-CD370 Purified CLEC9A Monoclonal Antibody (M04577)
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